Journal article
Electrical synaptic transmission requires a postsynaptic scaffolding protein
eLife, Vol.10, 66898
04/28/2021
DOI: 10.7554/eLife.66898
PMCID: PMC8081524
PMID: 33908867
Abstract
Electrical synaptic transmission relies on neuronal gap junctions containing channels constructed by Connexins. While at chemical synapses neurotransmitter-gated ion channels are critically supported by scaffolding proteins, it is unknown if channels at electrical synapses require similar scaffold support. Here, we investigated the functional relationship between neuronal Connexins and Zonula Occludens 1 (ZO1), an intracellular scaffolding protein localized to electrical synapses. Using model electrical synapses in zebrafish Mauthner cells, we demonstrated that ZO1 is required for robust synaptic Connexin localization, but Connexins are dispensable for ZO1 localization. Disrupting this hierarchical ZO1/Connexin relationship abolishes electrical transmission and disrupts Mauthner cell-initiated escape responses. We found that ZO1 is asymmetrically localized exclusively postsynaptically at neuronal contacts where it functions to assemble intercellular channels. Thus, forming functional neuronal gap junctions requires a postsynaptic scaffolding protein. The critical function of a scaffolding molecule reveals an unanticipated complexity of molecular and functional organization at electrical synapses.
Details
- Title: Subtitle
- Electrical synaptic transmission requires a postsynaptic scaffolding protein
- Creators
- Abagael M. Lasseigne - University of OregonFabio A. Echeverry - Albert Einstein College of MedicineSundas Ijaz - Albert Einstein College of MedicineJennifer Carlisle Michel - University of OregonE Anne Martin - University of OregonAudrey J. Marsh - University of OregonElisa Trujillo - University of OregonKurt C. Marsden - North Carolina State UniversityAlberto E. Pereda - Albert Einstein College of MedicineAdam C. Miller - University of Oregon
- Resource Type
- Journal article
- Publication Details
- eLife, Vol.10, 66898
- DOI
- 10.7554/eLife.66898
- PMID
- 33908867
- PMCID
- PMC8081524
- NLM abbreviation
- Elife
- ISSN
- 2050-084X
- eISSN
- 2050-084X
- Publisher
- Elife Sciences Publications Ltd
- Number of pages
- 38
- Grant note
- T32HD007348; F32HD102182 / Eunice Kennedy Shriver National Institute of Child Health and Human Development; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) Eunice Kennedy Shriver National Institute of Child Health and Human Development; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) RF1MH120016 / National Institute of Mental Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Mental Health (NIMH) R01DC011099 / National Institute on Deafness and Other Communication Disorders; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Deafness & Other Communication Disorders (NIDCD) R21NS085772; R21NS117967; R01NS105758 / National Institute of Neurological Disorders and Stroke; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS)
- Language
- English
- Date published
- 04/28/2021
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984937941702771
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