Electroconvulsive seizure behavior in Drosophila: analysis of the physiological repertoire underlying a stereotyped action pattern in bang-sensitive mutants

Jisue Lee; Chun-Fang Wu

doi:10.1523/JNEUROSCI.22-24-11065.2002

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Electroconvulsive seizure behavior in Drosophila: analysis of the physiological repertoire underlying a stereotyped action pattern in bang-sensitive mutants

Journal article

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Peer reviewed

Electroconvulsive seizure behavior in Drosophila: analysis of the physiological repertoire underlying a stereotyped action pattern in bang-sensitive mutants

Jisue Lee and Chun-Fang Wu

The Journal of neuroscience, Vol.22(24), pp.11065-11079

12/15/2002

DOI: 10.1523/JNEUROSCI.22-24-11065.2002

PMCID: PMC6758420

PMID: 12486202

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Abstract

Drosophila bang-sensitive mutants display a remarkable stereotyped behavioral sequence during mechanical disturbances. This seizure repertoire consists of initial and delayed bouts of spasm interposed with paralysis and followed by recovery of activity and a period of refractoriness to further stimulation. Electroconvulsive stimuli across the brain induced a similar seizure behavior in tethered flies, in which corresponding electrophysiological events could be readily recorded in indirect flight muscles [dorsal longitudinal muscles (DLMs)] of the giant fiber (GF) pathway. The DLM physiological repertoire consisted of initial and delayed discharges (IDs and DDs), a response failure and recovery, followed by a refractory period. Interestingly, wild-type flies also displayed the same electroconvulsive repertoire, albeit inducible only at higher stimulus intensities and with briefer expression. The DLM repertoire presumably originated from activities of distinct neural circuits subserving normal function and reflected the general sequence of excitation and depression of the nervous system as a whole, as shown by simultaneous recordings along the different body axes. The well characterized GF pathway facilitated localization of circuits responsible for response failure and ID and DD motor patterns by surgical manipulations, recording-stimulating site analysis, and genetic mosaic studies. A flight pattern generator is most likely the major contributor to shaping the DD pattern, with modifications by active integration of individual motor neurons and associated interneurons. The robust electroconvulsive repertoire of DLMs provides a convenient window for further genetic analysis of the interacting neural mechanisms underlying a stereotyped action pattern in Drosophila, which shows striking parallels with aspects of seizure in mammalian species.

Motor Neurons - physiology

Movement

Thorax - innervation

Stress, Mechanical

Muscle, Skeletal - innervation

Motor Activity

Neural Pathways

Drosophila - anatomy & histology

Drosophila - physiology

GABA Antagonists - pharmacology

Refractory Period, Electrophysiological

Action Potentials

Behavior, Animal

Animals

Nerve Fibers - physiology

Nervous System Physiological Phenomena

Stereotyped Behavior

Seizures - etiology

Kinetics

Models, Neurological

Mutation

Electroshock

Drosophila - genetics

Details

Title: Subtitle: Electroconvulsive seizure behavior in Drosophila: analysis of the physiological repertoire underlying a stereotyped action pattern in bang-sensitive mutants
Creators: Jisue Lee - Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USA
Chun-Fang Wu
Resource Type: Journal article
Publication Details: The Journal of neuroscience, Vol.22(24), pp.11065-11079
Publisher: United States
DOI: 10.1523/JNEUROSCI.22-24-11065.2002
PMID: 12486202
PMCID: PMC6758420
ISSN: 0270-6474
eISSN: 1529-2401
Grant note: NS18500 / NINDS NIH HHS NS28528 / NINDS NIH HHS
Language: English
Date published: 12/15/2002
Academic Unit: Iowa Neuroscience Institute; Biology
Record Identifier: 9984070421602771

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