Journal article
Electron currents generated by the human phagocyte NADPH oxidase
Nature (London), Vol.392(6677), pp.734-737
04/16/1998
DOI: 10.1038/33725
PMID: 9565037
Abstract
Electron transport across biological membranes is a well-known feature of bacteria, mitochondria and chloroplasts, where it provides motive forces for vectorial transport processes. In contrast, electron transport is generally not found in the plasma membrane of eukaryotic cells, possibly because it would interfere with electric processes at the plasma membrane. An exception is provided by the phagocyte NADPH oxidase, which generates superoxide () through electron transfer from cytosolic NADPH to extracellular oxygen. The enzyme is essential for host defence, and patients with chronic granulomatous disease, who lack the functional enzyme, suffer from severe infections,. It has been suggested that electron transfer by the NADPH oxidase might be electrogenic. Here we demonstrate, using the whole-cell patch-clamp technique, the generation of electron currents by the NADPH oxidase in human eosinophil granulocytes. The currents were absent in granulocytes of sufferers of chronic granulomatous disease and under conditions of low oxygen. Generation of electron currents across the plasma membrane of eukaryotic cells has not been observed previously and might be - independently of the generation of superoxide - a physiologically relevant function of the phagocyte NADPH oxidase.
Details
- Title: Subtitle
- Electron currents generated by the human phagocyte NADPH oxidase
- Creators
- Karl-Heinz Krause - Division of Infectious Diseases, Department of Medicine, University Hospital, SwitzerlandJacques Schrenzel - University Hospital of GenevaLena Serrander - Division of Infectious Diseases, Department of Medicine, University Hospital, SwitzerlandBotond Bánfi - Division of Infectious Diseases, Department of Medicine, University Hospital, SwitzerlandOliver Nüße - Division of Infectious Diseases, Department of Medicine, University HospitalReyhaneh Fouyouzi - Division of Infectious Diseases, Department of Medicine, University Hospital, SwitzerlandDaniel P Lew - Division of Infectious Diseases, Department of Medicine, University Hospital, SwitzerlandNicolas Demaurex - Department of Physiology, University Medical Center, Switzerland
- Resource Type
- Journal article
- Publication Details
- Nature (London), Vol.392(6677), pp.734-737
- DOI
- 10.1038/33725
- PMID
- 9565037
- ISSN
- 0028-0836
- eISSN
- 1476-4687
- Language
- English
- Date published
- 04/16/1998
- Academic Unit
- Anatomy and Cell Biology; Otolaryngology; Internal Medicine
- Record Identifier
- 9984284342702771
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