Journal article
Electrostatic Surface Properties of Blood and Semen Extracellular Vesicles: Implications of Sialylation and HIV-Induced Changes on EV Internalization
Viruses, Vol.12(10), p.1117
10/01/2020
DOI: 10.3390/v12101117
PMCID: PMC7601085
PMID: 33019624
Abstract
Although extracellular vesicle (EV) surface electrostatic properties (measured as zeta potential, zeta-potential) have been reported by many investigators, the biophysical implications of charge and EV origin remains uncertain. Here, we compared the zeta-potential of human blood EVs (BEVs) and semen EVs (SEVs) from 26 donors that were HIV-infected (HIV+, n = 13) or HIV uninfected (HIV-, n = 13). We found that, compared to BEVs that bear neutral surface charge, SEVs were significantly more negatively charged, even when BEVs and SEVs were from the same individual. Comparison of BEVs and SEVs from HIV- and HIV+ groups revealed subtle HIV-induced alteration in the zeta-potential of EVs, with the effect being more significant in SEVs ( increment zeta-potential = -8.82 mV, p-value = 0.0062) than BEVs ( increment zeta-potential = -1.4 mV, p-value = 0.0462). These observations were validated by differences in the isoelectric point (IEP) of EVs, which was in the order of HIV + SEV <= HIV-SEV MUCH LESS-THAN HIV + BEV <= HIV-BEV. Functionally, the rate and efficiency of SEV internalization by the human cervical epithelial cell line, primary peripheral blood lymphocytes, and primary blood-derived monocytes were significantly higher than those of BEVs. Mechanistically, removal of sialic acids from the surface of EVs using neuraminidase treatment significantly decreased SEV's surface charge, concomitant with a substantial reduction in SEV's internalization. The neuraminidase effect was independent of HIV infection and insignificant for BEVs. Finally, these results were corroborated by enrichment of glycoproteins in SEVs versus BEVs. Taken together, these findings uncover fundamental tissue-specific differences in surface electrostatic properties of EVs and highlight the critical role of surface charge in EV/target cell interactions.
Details
- Title: Subtitle
- Electrostatic Surface Properties of Blood and Semen Extracellular Vesicles: Implications of Sialylation and HIV-Induced Changes on EV Internalization
- Creators
- Hussein Kaddour - Stony Brook UniversityTyler D. Panzner - Stony Brook UniversityJennifer L. Welch - Veterans Health AdministrationNadia Shouman - Stony Brook UniversityMahesh Mohan - Texas Biomedical Research InstituteJack T. Stapleton - Veterans Health AdministrationChioma M. Okeoma - Stony Brook University
- Resource Type
- Journal article
- Publication Details
- Viruses, Vol.12(10), p.1117
- DOI
- 10.3390/v12101117
- PMID
- 33019624
- PMCID
- PMC7601085
- NLM abbreviation
- Viruses
- ISSN
- 1999-4915
- eISSN
- 1999-4915
- Publisher
- Mdpi
- Number of pages
- 22
- Grant note
- DA042348; 5T32AI007533-18 / National Institutes of Health (NIH); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA BX000207 / VA Merit Review; US Department of Veterans Affairs DA042348; DA050169 / National Institute on Drug Abuse (NIDA); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Drug Abuse (NIDA)
- Language
- English
- Date published
- 10/01/2020
- Academic Unit
- Microbiology and Immunology; Infectious Diseases; Internal Medicine
- Record Identifier
- 9984297332202771
Metrics
18 Record Views