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Electrostatic dust collectors compared to inhalable samplers for measuring endotoxin concentrations in farm homes
Journal article   Open access

Electrostatic dust collectors compared to inhalable samplers for measuring endotoxin concentrations in farm homes

Brita Kilburg-Basnyat, Thomas M Peters, Sarah S Perry and Peter S Thorne
Indoor air, Vol.26(5), pp.724-733
10/2016
DOI: 10.1111/ina.12243
PMCID: PMC4850132
PMID: 26296624
url
https://doi.org/10.1111/ina.12243View
Published (Version of record) Open Access

Abstract

Paired electrostatic dust collectors (EDCs) and daily, inhalable button samplers (BS) were used concurrently to sample endotoxin in 10 farm homes during 7-day periods in summer and winter. Winter sampling included an optical particle counter (OPC) to measure PM 2.5 and PM 2.5-10 . Electrostatic dust collectors and BS filters were analyzed for endotoxin using the kinetic chromogenic Limulus amebocyte lysate assay. Optical particle counter particulate matter (PM) data were divided into two PM categories. In summer, geometric mean (geometric standard deviation) endotoxin concentrations were 0.82 EU/m 3 (2.7) measured with the BS and 737 EU/m 2 (1.9) measured with the EDC. Winter values were 0.52 EU/m 3 (3.1) for BS and 538 EU/m 2 (3.0) for EDCs. Seven day endotoxin values of EDCs were highly correlated with the 7-day BS sampling averages (r=0.70; p <0.001). Analysis of variance indicated a 2.4-fold increase in EDC endotoxin concentrations for each unit increase of the ratio of PM 2.5 to PM 2.5-10. There was also a significant correlation between BS and EDCs endotoxin concentrations for winter (r=0.67; p <0.05) and summer(r=0.75; p <0.05). Thus, EDCs sample comparable endotoxin concentrations to BS, making EDCs a feasible, easy to use alternative to BS for endotoxin sampling.
optical particle counter electrostatic dust collector lipopolysaccharide bioaerosol button sampler

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