Journal article
Elevated Levels of Glucose and L‐Fucose Reduce 22Na+ Uptake and Whole Cell Na+ Current in Cultured Neuroblastoma Cells
Journal of neurochemistry, Vol.62(1), pp.63-69
01/1994
DOI: 10.1046/j.1471-4159.1994.62010063.x
PMID: 8263545
Abstract
: Na+ flux was studied in cultured neuroblastoma cells grown in medium containing increased glucose or L‐fucose concentrations. Chronic exposure of neuroblastoma cells to 30 mM glucose or 30 mM L‐fucose caused a decrease in ouabain‐sensitive and veratridine‐stimulated 22Na+ uptake compared with cells cultured in unsupplemented medium. The Na+ current, determined by using whole‐cell configuration of the patch clamp, was also decreased in these cells. Tetrodotoxin (3 μM), which blocked whole cell Na+ currents, also blocked veratridine‐stimulated 22Na+ accumulation. Culturing cells in medium containing 30 mM fructose as an osmotic control had no effect on Na+ flux. Specific [3H] saxitoxin binding was not affected by 30 mM glucose or 30 mM L‐fucose compared with cells grown in unsupplemented medium, suggesting that the number of Na+ channels was not decreased. These studies suggest that exposing cultured neuronal cells to conditions that occur in the diabetic milieu alters Na+ transport and Na+‐channel activity.
Details
- Title: Subtitle
- Elevated Levels of Glucose and L‐Fucose Reduce 22Na+ Uptake and Whole Cell Na+ Current in Cultured Neuroblastoma Cells
- Creators
- Mark A YorekMark R StefaniRuth E Wachtel
- Resource Type
- Journal article
- Publication Details
- Journal of neurochemistry, Vol.62(1), pp.63-69
- Publisher
- Blackwell Publishing Ltd
- DOI
- 10.1046/j.1471-4159.1994.62010063.x
- PMID
- 8263545
- ISSN
- 0022-3042
- eISSN
- 1471-4159
- Number of pages
- 7
- Language
- English
- Date published
- 01/1994
- Academic Unit
- Anesthesia; Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984094588202771
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