Elevated Notch ligands in serum are associated with HIV/TB coinfection

Jennifer R. Bermick; Pamela M. Lincoln; Ronald M. Allen; Steven L. Kunkel; Matthew A. Schaller

doi:10.1016/j.jctube.2021.100258

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Elevated Notch ligands in serum are associated with HIV/TB coinfection

Journal article

Open access

Peer reviewed

Elevated Notch ligands in serum are associated with HIV/TB coinfection

Jennifer R. Bermick, Pamela M. Lincoln, Ronald M. Allen, Steven L. Kunkel and Matthew A. Schaller

Journal of clinical tuberculosis and other mycobacterial diseases, Vol.24, p.100258

08/2021

DOI: 10.1016/j.jctube.2021.100258

PMCID: PMC8258674

PMID: 34307905

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Abstract

There is a clear need for improved biomarkers to diagnose HIV/TB coinfection. Although numerous tests can identify the existence of both of these microbes within the host, a parallel assessment of the host response to HIV/TB coinfection may prove as useful confirmation in cases where microbiological tests are inconclusive. To this end we assessed the levels of Notch ligands found in serum samples of patients with TB, HIV or HIV/TB coinfection. The Notch system is involved in almost every stage of development, including the maturation of the immune response. Upon exposure to a pathogen, the innate immune system will increase expression of Notch ligands Delta-like 1 and Delta-like 4. Previous research has demonstrated that Notch ligand expression is increased on monocytes from patients diagnosed with tuberculosis. We hypothesized that if Notch ligands were present in the peripheral blood of individuals diagnosed with TB, they may serve as a novel marker for infection. Design: Serum samples from patients with HIV, TB or HIV/TB coinfection were compared to serum from uninfected individuals to determine levels of DLL1 and DLL4 in a case controlled study. DLL1 and DLL4 were measured by ELISA. Linear regression with post tests were used to determine if levels of DLL1 and DLL4 were increased in individuals with HIV/TB coinfection as compared to individuals infected with either HIV or TB or healthy controls. Delta-like 1 and Delta-like 4 were significantly increased in the serum of patients with HIV and HIV/ M. tuberculosis coinfection compared to other groups. Assessment of Notch ligands in peripheral blood may enhance the diagnosis of individuals with active TB that are co-infected with HIV. The study will also need to be validated in in a larger cohort.

Biomarker

HIV

Mycobacterium tuberculosis

Notch ligands

Details

Title: Subtitle: Elevated Notch ligands in serum are associated with HIV/TB coinfection
Creators: Jennifer R. Bermick - University of Iowa
Pamela M. Lincoln - University of Michigan
Ronald M. Allen - University of Michigan
Steven L. Kunkel - University of Michigan
Matthew A. Schaller - University of Michigan
Resource Type: Journal article
Publication Details: Journal of clinical tuberculosis and other mycobacterial diseases, Vol.24, p.100258
DOI: 10.1016/j.jctube.2021.100258
PMID: 34307905
PMCID: PMC8258674
NLM abbreviation: J Clin Tuberc Other Mycobact Dis
ISSN: 2405-5794
eISSN: 2405-5794
Publisher: Elsevier Ltd
Grant note: DOI: 10.13039/100000050, name: National Heart Lung and Blood Institute, award: NIH 5R01HL03123732, U044689; DOI: 10.13039/100007270, name: University of Michigan, award: NIH 5R01HL03123732, U044689; DOI: 10.13039/100008269, name: Michigan Institute for Clinical and Health Research, award: NIH 5R01HL03123732, U044689
Language: English
Date published: 08/2021
Academic Unit: Stead Family Department of Pediatrics; Neonatology
Record Identifier: 9984353933802771

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