Journal article
Elevated levels of homocysteine compromise blood-brain barrier integrity in mice
Blood, Vol.107(2), pp.591-593
01/15/2006
DOI: 10.1182/blood-2005-06-2506
PMCID: PMC1895614
PMID: 16189268
Abstract
Elevated levels of plasma homocysteine (Hcy) correlate with increased risk of cardiovascular and Alzheimer diseases. We studied the effect of elevated Hcy on the blood-brain barrier (BBB) to explore the possibility of a vascular link between the 2 diseases. On a hyperhomocysteinemic diet, cystathionine beta-synthase (Cbs)-heterozygous mice develop hyperhomocysteinemia. Intravital microscopy analysis of the mesenteric venules showed that leukocyte rolling velocity was markedly decreased and numbers of adherent cells were increased in the mutant mice. This was due at least in part to increased expression of P-selectin. BBB permeability was measured by Evans blue dye permeation and was found to be 25% greater in the Cbs(+/-) cortex compared with wild-type controls. Our study suggests an important toxic effect of elevated Hcy on brain microvessels and implicates Hcy in the disruption of the BBB.
Details
- Title: Subtitle
- Elevated levels of homocysteine compromise blood-brain barrier integrity in mice
- Creators
- Atul F Kamath - CBR Institute for Biomedical Research, 800 Huntington Ave, Boston, MA 02115, USAAnil K ChauhanJanka KisuckaVandana S DoleJoseph LoscalzoDiane E HandyDenisa D Wagner
- Resource Type
- Journal article
- Publication Details
- Blood, Vol.107(2), pp.591-593
- DOI
- 10.1182/blood-2005-06-2506
- PMID
- 16189268
- PMCID
- PMC1895614
- ISSN
- 0006-4971
- eISSN
- 1528-0020
- Grant note
- R37 HL41002 / NHLBI NIH HHS R01 HL041002 / NHLBI NIH HHS HL58976 / NHLBI NIH HHS
- Language
- English
- Date published
- 01/15/2006
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984094343702771
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