Elevated membrane attack complex in human choroid with high risk complement factor H genotypes

Robert F Mullins; Aaron D Dewald; Luan M Streb; Kai Wang; Markus H Kuehn; Edwin M Stone

doi:10.1016/j.exer.2011.06.015

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Elevated membrane attack complex in human choroid with high risk complement factor H genotypes

Journal article

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Peer reviewed

Elevated membrane attack complex in human choroid with high risk complement factor H genotypes

Robert F Mullins, Aaron D Dewald, Luan M Streb, Kai Wang, Markus H Kuehn and Edwin M Stone

Experimental Eye Research, Vol.93(4), pp.565-567

2011

DOI: 10.1016/j.exer.2011.06.015

PMCID: PMC3206185

PMID: 21729696

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https://www.ncbi.nlm.nih.gov/pmc/articles/3206185View

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Abstract

Data from human genetics, histopathology, and animal models reveal a major role for the complement system in the development of age-related macular degeneration (AMD). Genetic variations in the complement factor H ( CFH) gene are associated with an elevated risk of AMD. In this study we sought to determine whether eyes from donors with a high-risk genotype (homozygosity for the histidine allele at codon 402) exhibit altered levels of membrane attack complex (MAC) in the choroid, compared to eyes with a low risk genotype (homozygosity for tyrosine). Proteins were extracted from the RPE/choroid of 18 donors (10 low risk and 8 high risk) and levels of MAC were assessed using an ELISA assay. Eyes from donors homozygous for the histidine allele showed 69% higher levels of MAC than those homozygous for the tyrosine allele ( p < 0.05), independent of whether the eyes showed signs of early AMD. Our results provide evidence that high-risk CFH genotypes may affect AMD risk by increased deposition of MAC around the aging choriocapillaris.

choroid

complement

choriocapillaris

macular degeneration

Details

Title: Subtitle: Elevated membrane attack complex in human choroid with high risk complement factor H genotypes
Creators: Robert F Mullins
Aaron D Dewald
Luan M Streb
Kai Wang
Markus H Kuehn
Edwin M Stone
Resource Type: Journal article
Publication Details: Experimental Eye Research, Vol.93(4), pp.565-567
DOI: 10.1016/j.exer.2011.06.015
PMID: 21729696
PMCID: PMC3206185
NLM abbreviation: Exp Eye Res
ISSN: 0014-4835
eISSN: 1096-0007
Publisher: Elsevier Ltd
Grant note: EY017451; 016822 / NEI Hansjoerg E.J.W. Kolder Professorship for Best Disease Research Macula Vision Research Foundation Foundation Fighting Blindness Howard Hughes Medical Institute
Language: English
Date published: 2011
Academic Unit: The University of Iowa Institute for Vision Research; Iowa Neuroscience Institute; Biostatistics; Ophthalmology and Visual Sciences
Record Identifier: 9983980064902771

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