Journal article
Elevated mutant frequencies in gene lacI in splenic lipopolysaccharide blasts after exposure to activated phagocytes in vitro
European journal of immunology, Vol.27(9), pp.2160-2164
09/1997
DOI: 10.1002/eji.1830270907
PMID: 9341754
Abstract
The interaction of B lymphocytes with phagocytes is critical for shaping the humoral immune response, as well as various aspects of normal and malignant B cell development, and has therefore been studied by immunologists in great detail. However, one potential outcome of this confrontation is often neglected, namely the mutagenicity of phagocytes to B lymphocytes. We are interested in phagocyte‐induced B cell mutagenesis and have conducted a feasibility study on the utility of a transgenic reporter assay to evaluate mutant frequencies in B cells that have encountered phagocytes. An in vitro co‐incubation system was designed in which splenic lipopolysaccharide (LPS) blasts carrying a phage Δ‐derived lacI transgene were exposed to pristane‐elicited peritoneal exudate cells (PEC). Mutant frequencies in LPS blasts were significantly increased (up to 6‐fold) when the cells were co‐incubated with PEC that had been stimulated by phorbol myristate acetate to undergo an oxidative burst. The lacI‐based transgenic mutation assay proved also useful for assessing mutagenicity in vivo, as demonstrated by the detection of elevated mutant frequencies in the spleen (3‐fold) and the inflammatory granuloma (4.7‐fold) obtained from pristane‐treated mice. We propose to utilize the lacI‐based transgenic mutagenesis assay as a tool to evaluate mutational levels during normal and aberrant B cell differentiation.
Details
- Title: Subtitle
- Elevated mutant frequencies in gene lacI in splenic lipopolysaccharide blasts after exposure to activated phagocytes in vitro
- Creators
- Klaus FelixSophia LinSiegfried Janz
- Resource Type
- Journal article
- Publication Details
- European journal of immunology, Vol.27(9), pp.2160-2164
- Publisher
- WILEY‐VCH Verlag GmbH
- DOI
- 10.1002/eji.1830270907
- PMID
- 9341754
- ISSN
- 0014-2980
- eISSN
- 1521-4141
- Number of pages
- 5
- Grant note
- The Mildred‐Scheel‐Stiftung
- Language
- English
- Date published
- 09/1997
- Academic Unit
- Pathology
- Record Identifier
- 9984083250602771
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