Elevated mutant frequencies in lymphoid tissues persist throughout plasmacytoma development in BALB/c.lambdaLIZ mice

K Felix; K A Kelliher; G W Bornkamm; S Janz

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Elevated mutant frequencies in lymphoid tissues persist throughout plasmacytoma development in BALB/c.lambdaLIZ mice

Journal article

Peer reviewed

Elevated mutant frequencies in lymphoid tissues persist throughout plasmacytoma development in BALB/c.lambdaLIZ mice

K Felix, K A Kelliher, G W Bornkamm and S Janz

Cancer research (Chicago, Ill.), Vol.59(15), pp.3621-3626

08/01/1999

PMID: 10446972

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Abstract

Using the phage lambdaLIZ-based transgenic in vivo mutagenesis assay, the mean mutant frequencies in the target gene, lacI, were found to be significantly increased in lymphoid tissues of congenic BALB/c.lambdaLIZ N5 mice in the terminal stage of a plasmacytoma induction experiment, 213-280 days after the first i.p. injection of the plasmacytomagenic agent pristane (2,6,10,14-tetramethylpentadecane). In plasmacytoma-bearing mice (n = 7), mutant frequencies in the spleens and mesenteric lymph nodes were elevated 2.46-fold and 5.35-fold, respectively, when compared with age-matched controls. In plasmacytoma-negative mice (n = 11), mutant frequencies were increased 2.30-fold (spleens) and 3.48-fold (mesenteric nodes). These results, interpreted in conjunction with our previous findings (K. Felix et al., Cancer Res., 58: 1616-1619, 1998) of approximately 3-fold elevations in pristane-induced splenic mutagenesis on day 42 postpristane, indicate that increased mutant levels in lymphoid tissues persist throughout plasmacytomagenesis in genetically susceptible BALB/c mice.

Lymph Nodes - pathology

Plasmacytoma - chemically induced

Plasmacytoma - genetics

Lac Operon - genetics

Male

Terpenes - toxicity

Plasmacytoma - pathology

Spleen - drug effects

Carcinogens - toxicity

DNA Mutational Analysis

Female

Lymph Nodes - drug effects

Spleen - pathology

Lymphoid Tissue - drug effects

Peritoneal Neoplasms - pathology

Lymph Nodes - chemistry

Mice, Inbred C57BL

Bacterial Proteins - genetics

Lac Repressors

Peritoneal Neoplasms - chemically induced

Repressor Proteins - genetics

Genes, Reporter - genetics

Mice, Transgenic

Disease Progression

Spleen - chemistry

DNA - genetics

Lymphoid Tissue - pathology

Lymphoid Tissue - chemistry

Animals

Escherichia coli Proteins

Mutagenesis

Peritoneal Neoplasms - genetics

Mice

Mice, Inbred BALB C

DNA, Neoplasm - genetics

Details

Title: Subtitle: Elevated mutant frequencies in lymphoid tissues persist throughout plasmacytoma development in BALB/c.lambdaLIZ mice
Creators: K Felix - Division of Basic Sciences, National Cancer Institute, NIH, Bethesda, Maryland 20892-4255, USA. felixk@dc37a.nci.nih.gov
K A Kelliher
G W Bornkamm
S Janz
Resource Type: Journal article
Publication Details: Cancer research (Chicago, Ill.), Vol.59(15), pp.3621-3626
PMID: 10446972
ISSN: 0008-5472
eISSN: 1538-7445
Language: English
Date published: 08/01/1999
Academic Unit: Pathology
Record Identifier: 9984083860802771

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