Journal article
Elongator complex is required for long-term olfactory memory formation in Drosophila
Learning & memory (Cold Spring Harbor, N.Y.), Vol.25(4), pp.183-196
04/2018
DOI: 10.1101/lm.046557.117
PMCID: PMC5855525
PMID: 29545390
Abstract
The evolutionarily conserved Elongator Complex associates with RNA polymerase II for transcriptional elongation. Elp3 is the catalytic subunit, contains histone acetyltransferase activity, and is associated with neurodegeneration in humans. Elp1 is a scaffolding subunit and when mutated causes familial dysautonomia. Here, we show that
and
are required for aversive long-term olfactory memory in
RNAi knockdown of
in adult mushroom bodies impairs long-term memory (LTM) without affecting earlier forms of memory. RNAi knockdown with coexpression of
cDNA reverses the impairment. Similarly, RNAi knockdown of
impairs LTM and coexpression of
cDNA reverses this phenotype. The LTM deficit in
and
knockdown flies is accompanied by the abolishment of a LTM trace, which is registered as increased calcium influx in response to the CS+ odor in the α-branch of mushroom body neurons. Coexpression of
or
cDNA rescues the memory trace in parallel with LTM. These data show that the Elongator complex is required in adult mushroom body neurons for long-term behavioral memory and the associated long-term memory trace.
Details
- Title: Subtitle
- Elongator complex is required for long-term olfactory memory formation in Drosophila
- Creators
- Dinghui Yu - Baylor College of MedicineYing Tan - Baylor College of MedicineMolee Chakraborty - Scripps Research InstituteSeth Tomchik - Scripps Research InstituteRonald L Davis - Scripps Research Institute
- Resource Type
- Journal article
- Publication Details
- Learning & memory (Cold Spring Harbor, N.Y.), Vol.25(4), pp.183-196
- DOI
- 10.1101/lm.046557.117
- PMID
- 29545390
- PMCID
- PMC5855525
- NLM abbreviation
- Learn Mem
- ISSN
- 1072-0502
- eISSN
- 1549-5485
- Grant note
- U54 HD083092 / NICHD NIH HHS R35 NS097224 / NINDS NIH HHS R37 NS019904 / NINDS NIH HHS R01 NS052351 / NINDS NIH HHS
- Language
- English
- Date published
- 04/2018
- Academic Unit
- Iowa Neuroscience Institute; Neuroscience and Pharmacology
- Record Identifier
- 9984303746402771
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