Embryo blastulation and quality between days 5 and 6 of extended embryo culture

E B Nguyen; E A Jacobs; K M Summers; A E Sparks; B J Van Voorhis; V E Klenov; E H Duran

doi:10.1007/s10815-021-02156-7

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Embryo blastulation and quality between days 5 and 6 of extended embryo culture

Journal article

Open access

Peer reviewed

Embryo blastulation and quality between days 5 and 6 of extended embryo culture

E B Nguyen, E A Jacobs, K M Summers, A E Sparks, B J Van Voorhis, V E Klenov and E H Duran

Journal of assisted reproduction and genetics, Vol.38(8), pp.2193-2198

08/2021

DOI: 10.1007/s10815-021-02156-7

PMCID: PMC8417162

PMID: 33754252

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417162View

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Abstract

This study aims to know what proportion of culture day 5 pre-blastocyst-stage embryos develop into blastocysts by culture day 6 and what patient and cycle characteristics are associated with delayed blastocyst formation. A retrospective observational cohort analysis was performed including a total of 9886 embryos from 1008 IVF cycles in 835 patients, who underwent treatment between January 1, 2016, and December 31, 2018. Autologous fresh in vitro fertilization (IVF) cycles at a single academic center were included in the analysis. Embryos were group-cultured using single-step culture media. Blastulation was defined as the presence of a new blastocyst. Usable blastulation was defined as the presence of a new good or excellent quality, expanded, hatching, or hatched blastocysts. The mean blastulation rate between days 5 and 6 of extended embryo culture was 30.9%. The mean percentage of embryos developing into usable blastocyst-stage embryos was 19.8%. The factors associated with blastulation on day 6 included the total number of embryos and the number of pre-blastocysts on day 5, as well as the use of ICSI. Age, the number of total embryos, those remained in culture and pre-blastocysts, as well as the blastulation rate on day 5 were associated with usable blastulation. It is important to know the usable blastocyst development rate between culture days 5 and 6 in order to adequately counsel patients debating whether to proceed with fresh ET on day 5 or forego ET with the expectation that embryos will be biopsied for PGT and/or cryopreserved on culture day 6. Our findings provide evidence to help guide patients in this difficult decision.

Adult

Blastocyst - cytology

Cryopreservation

Embryo Culture Techniques - methods

Embryo Implantation

Embryo Transfer

Embryo, Mammalian - cytology

Female

Fertilization in Vitro - methods

Humans

Pregnancy

Pregnancy Rate

Retrospective Studies

Details

Title: Subtitle: Embryo blastulation and quality between days 5 and 6 of extended embryo culture
Creators: E B Nguyen - University of Iowa Hospitals and Clinics
E A Jacobs - University of Iowa Hospitals and Clinics
K M Summers - University of Iowa
A E Sparks - University of Iowa
B J Van Voorhis - University of Iowa
V E Klenov - University of Iowa
E H Duran - University of Iowa Hospitals and Clinics
Resource Type: Journal article
Publication Details: Journal of assisted reproduction and genetics, Vol.38(8), pp.2193-2198
DOI: 10.1007/s10815-021-02156-7
PMID: 33754252
PMCID: PMC8417162
ISSN: 1058-0468
eISSN: 1573-7330
Language: English
Date published: 08/2021
Academic Unit: Obstetrics and Gynecology
Record Identifier: 9984318221002771

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