Journal article
Emi2 enables centriole amplification during multiciliated cell differentiation
Science advances, Vol.8(13), pp.eabm7538-eabm7538
04/2022
DOI: 10.1126/sciadv.abm7538
PMCID: PMC10938574
PMID: 35363516
Abstract
Massive centriole amplification during multiciliated cell (MCC) differentiation is a notable example of organelle biogenesis. This process is thought to be enabled by a derived cell cycle state, but the key cell cycle components required for centriole amplification in MCC progenitors remain poorly defined. Here, we show that
emi2
(
fbxo43
) expression is up-regulated and acts in MCC progenitors after cell cycle exit to transiently inhibit anaphase-promoting complex/cyclosome (APC/C)
cdh1
activity. We find that this inhibition is required for the phosphorylation and activation of a key cell cycle kinase, plk1, which acts, in turn, to promote different steps required for centriole amplification and basal body formation, including centriole disengagement, apical migration, and maturation into basal bodies. This emi2-APC/C-plk1 axis is also required to down-regulate gene expression essential for centriole amplification after differentiation is complete. These results identify an emi2-APC/C-plk1 axis that promotes and then terminates centriole assembly and basal body formation during MCC differentiation.
Centriole amplification and basal body production during MCC differentiation are regulated by an emi2-APC/C-plk1 axis.
Details
- Title: Subtitle
- Emi2 enables centriole amplification during multiciliated cell differentiation
- Creators
- Seongjae Kim - Salk Institute for Biological StudiesYuan-Hung Chien - Salk Institute for Biological StudiesAmy Ryan - University of Southern CaliforniaChris Kintner - Salk Institute for Biological Studies
- Resource Type
- Journal article
- Publication Details
- Science advances, Vol.8(13), pp.eabm7538-eabm7538
- DOI
- 10.1126/sciadv.abm7538
- PMID
- 35363516
- PMCID
- PMC10938574
- ISSN
- 2375-2548
- eISSN
- 2375-2548
- Language
- English
- Date published
- 04/2022
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984284456302771
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