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Emi2 enables centriole amplification during multiciliated cell differentiation
Journal article   Open access   Peer reviewed

Emi2 enables centriole amplification during multiciliated cell differentiation

Seongjae Kim, Yuan-Hung Chien, Amy Ryan and Chris Kintner
Science advances, Vol.8(13), pp.eabm7538-eabm7538
04/2022
DOI: 10.1126/sciadv.abm7538
PMCID: PMC10938574
PMID: 35363516
url
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10938574/View
Published (Version of record) Open Access

Abstract

Massive centriole amplification during multiciliated cell (MCC) differentiation is a notable example of organelle biogenesis. This process is thought to be enabled by a derived cell cycle state, but the key cell cycle components required for centriole amplification in MCC progenitors remain poorly defined. Here, we show that emi2 ( fbxo43 ) expression is up-regulated and acts in MCC progenitors after cell cycle exit to transiently inhibit anaphase-promoting complex/cyclosome (APC/C) cdh1 activity. We find that this inhibition is required for the phosphorylation and activation of a key cell cycle kinase, plk1, which acts, in turn, to promote different steps required for centriole amplification and basal body formation, including centriole disengagement, apical migration, and maturation into basal bodies. This emi2-APC/C-plk1 axis is also required to down-regulate gene expression essential for centriole amplification after differentiation is complete. These results identify an emi2-APC/C-plk1 axis that promotes and then terminates centriole assembly and basal body formation during MCC differentiation. Centriole amplification and basal body production during MCC differentiation are regulated by an emi2-APC/C-plk1 axis.

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