Enantiomeric enrichment of 2,2',3,3',6,6'-hexachlorobiphenyl (PCB 136) in mice after induction of CYP enzymes

Izabela Kania-Korwel; Wei Xie; Keri C Hornbuckle; Larry W Robertson; Hans-Joachim Lehmler

doi:10.1007/s00244-007-9111-4

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Enantiomeric enrichment of 2,2',3,3',6,6'-hexachlorobiphenyl (PCB 136) in mice after induction of CYP enzymes

Journal article

Peer reviewed

Enantiomeric enrichment of 2,2',3,3',6,6'-hexachlorobiphenyl (PCB 136) in mice after induction of CYP enzymes

Izabela Kania-Korwel, Wei Xie, Keri C Hornbuckle, Larry W Robertson and Hans-Joachim Lehmler

Archives of environmental contamination and toxicology, Vol.55(3), pp.510-517

10/2008

DOI: 10.1007/s00244-007-9111-4

PMID: 18437444

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Abstract

Several PCB congeners, present in commercial PCB formulations, are chiral. These PCBs can undergo enantiomeric enrichment in many animal species and in humans due to currently uncharacterized enantioselective biotransformation processes. To investigate if certain cytochrome P-450 enzymes (CYPs), such as CYP2B's, are responsible for this enantiomeric enrichment, we investigated the enantioselective disposition of (+/-)-PCB 136 in female mice after induction of different CYP enzymes by pretreatment with corn oil alone, beta-naphthoflavone (CYP1A's), phenobarbital (CYP2B's), or dexamethasone (2B's and 3A's), followed by oral PCB administration. PCB 136 levels were significantly lower in phenobarbital- and, to a lesser extent, in dexamethasone-pretreated animals, presumably due to the induction of PCB 136 metabolizing enzymes. Although (+)-PCB 136 was enriched in all tissues, none of the pretreatments altered the enantioselective disposition of PCB 136 in a manner that suggests a particular CYP subfamily as the cause of the enrichment of (+)-PCB 136. Fecal PCB levels and enantiomeric fraction values changed over time in a manner consistent with slower digestive motility in the mice pretreated with phenobarbital and dexamethasone. Overall, this study does not support the hypothesis that metabolism by CYP2B enzymes is responsible for the enrichment of (+)-PCB 136 in mice.

Tissue Distribution

Animals

Polychlorinated Biphenyls - chemistry

Stereoisomerism

Biotransformation

Mice, Inbred C57BL

Cytochrome P-450 Enzyme System - biosynthesis

Enzyme Induction

Female

Polychlorinated Biphenyls - pharmacokinetics

Mice

Chromatography, Gas

Details

Title: Subtitle: Enantiomeric enrichment of 2,2',3,3',6,6'-hexachlorobiphenyl (PCB 136) in mice after induction of CYP enzymes
Creators: Izabela Kania-Korwel - Department of Occupational and Environmental Health, University of Iowa, College of Public Health, 100 Oakdale Campus, 114 IREH, Iowa City, IA 52242, USA
Wei Xie
Keri C Hornbuckle
Larry W Robertson
Hans-Joachim Lehmler
Resource Type: Journal article
Publication Details: Archives of environmental contamination and toxicology, Vol.55(3), pp.510-517
DOI: 10.1007/s00244-007-9111-4
PMID: 18437444
NLM abbreviation: Arch Environ Contam Toxicol
ISSN: 0090-4341
eISSN: 1432-0703
Publisher: United States
Grant note: P30 ES005605 / NIEHS NIH HHS ES012475 / NIEHS NIH HHS P42 ES013661 / NIEHS NIH HHS ES05605 / NIEHS NIH HHS ES013661 / NIEHS NIH HHS
Language: English
Date published: 10/2008
Academic Unit: Civil and Environmental Engineering; Occupational and Environmental Health; Iowa Neuroscience Institute; Iowa Superfund Research Program
Record Identifier: 9983997432102771

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