Journal article
Enantioselective oxidation by a cyclohexanone monooxygenase from the xenobiotic-degrading Polaromonas sp. strain JS666
Journal of molecular catalysis. B, Enzymatic, Vol.78, pp.105-110
06/2012
DOI: 10.1016/j.molcatb.2012.03.002
Abstract
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► A cyclohexanone monooxygenase from Polaromonas strain JS666 was cloned into Escherichia coli. ► Strain JS666 degrades cis-dichloroethene and many other xenobiotic compounds. ► This CHMO was highly enantioselective when oxidizing 2-substituted cyclohexanones. ► No activity on cis-dichloroethene was observed (contrary to a previous hypothesis).
A cyclohexanone monooxygenase (CHMO) from the xenobiotic-degrading Polaromonas sp. strain JS666 was heterologously expressed in Escherichia coli, and its ability to catalyze enantio- and regiodivergent oxidations of prochiral and racemic ketones was investigated. The expression system was also used to evaluate this enzyme's potential role in the oxidation of cis-1,2-dichloroethene (cDCE), a groundwater pollutant for which strain JS666 is the only known assimilator. The substrate enantiopreference and -selectivity of the strain JS666 CHMO is similar to that of other CHMO-type enzymes; of note is this enzyme's excellent stereodiscrimination of 2-substituted cyclic ketones. The expression system exhibits no activity with ethene or cDCE as substrates under the tested conditions. Phylogenetic analysis shows that sequence variability among cyclohexanone monooxygenases could be a rich source of new enzyme activities and attributes.
Details
- Title: Subtitle
- Enantioselective oxidation by a cyclohexanone monooxygenase from the xenobiotic-degrading Polaromonas sp. strain JS666
- Creators
- Anne K Alexander - The University of Iowa, Department of Civil and Environmental Engineering, 4105 Seamans Center, Iowa City, IA 52242, USADavid Biedermann - Vienna University of Technology, Institute of Applied Synthetic Chemistry, Getreidemarkt 9/163-OC, A-1060 Vienna, AustriaMichael J Fink - Vienna University of Technology, Institute of Applied Synthetic Chemistry, Getreidemarkt 9/163-OC, A-1060 Vienna, AustriaMarko D Mihovilovic - Vienna University of Technology, Institute of Applied Synthetic Chemistry, Getreidemarkt 9/163-OC, A-1060 Vienna, AustriaTimothy E Mattes - The University of Iowa, Department of Civil and Environmental Engineering, 4105 Seamans Center, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Journal of molecular catalysis. B, Enzymatic, Vol.78, pp.105-110
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.molcatb.2012.03.002
- ISSN
- 1381-1177
- eISSN
- 1873-3158
- Language
- English
- Date published
- 06/2012
- Academic Unit
- Civil and Environmental Engineering
- Record Identifier
- 9983992084102771
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