Endogenous Interleukin-10 Inhibits Angiotensin II-Induced Vascular Dysfunction

Sean P Didion; Dale A Kinzenbaw; Laura I Schrader; Yi Chu; Frank M Faraci

doi:10.1161/HYPERTENSIONAHA.109.137158

Back

Endogenous Interleukin-10 Inhibits Angiotensin II-Induced Vascular Dysfunction

Journal article

Open access

Peer reviewed

Endogenous Interleukin-10 Inhibits Angiotensin II-Induced Vascular Dysfunction

Sean P Didion, Dale A Kinzenbaw, Laura I Schrader, Yi Chu and Frank M Faraci

Hypertension (Dallas, Tex. 1979), Vol.54(3), pp.619-624

09/2009

DOI: 10.1161/HYPERTENSIONAHA.109.137158

PMCID: PMC2861503

PMID: 19620507

Files and links (1)

url

https://doi.org/10.1161/HYPERTENSIONAHA.109.137158View

Published (Version of record) Open Access

Abstract

Angiotensin II (Ang II) produces inflammation and endothelial dysfunction in blood vessels. We tested the hypothesis that interleukin 10 (IL-10), an anti-inflammatory cytokine, protects against Ang II-induced vascular dysfunction. Responses of carotid arteries from wild-type and IL-10 deficient mice ( IL-10 −/− ) were examined in vitro following overnight incubation with vehicle or Ang II (1 nmol/L). In arteries from wild-type mice, acetylcholine (an endothelium-dependent agonist) produced relaxation that was not affected by Ang II. In contrast, relaxation to acetylcholine in arteries from IL-10 −/− mice was reduced by >50% by Ang II (P<0.05) and this effect was prevented by a scavenger of superoxide. Vascular superoxide increased ~2-fold (P<0.05) following treatment with Ang II in IL-10 −/− mice but not in wild-type. Following systemic administration of Ang II (1.4 mg/kg per day for 10 days), Ang II produced modest impairment of endothelial function in wild-type mice but marked impairment in IL-10 −/− mice (P<0.05) that was reversed by a superoxide scavenger. Increases in arterial pressure in response to Ang II were similar in wild-type and IL-10 −/− mice. These findings provide the first evidence that endogenous IL-10 limits Ang II-mediated oxidative stress and vascular dysfunction both in vitro and in vivo suggesting that at least some of the protective effects of IL-10 may occur within the vessel wall.

endothelium

genetically-altered mice

inflammation

carotid artery

oxidative stress

Details

Title: Subtitle: Endogenous Interleukin-10 Inhibits Angiotensin II-Induced Vascular Dysfunction
Creators: Sean P Didion - Department of Internal Medicine Division of Cardiovascular Diseases Carver College of Medicine Cardiovascular Center University of Iowa Iowa City, IA 52242
Dale A Kinzenbaw - Department of Internal Medicine Division of Cardiovascular Diseases Carver College of Medicine Cardiovascular Center University of Iowa Iowa City, IA 52242
Laura I Schrader - Department of Internal Medicine Division of Cardiovascular Diseases Carver College of Medicine Cardiovascular Center University of Iowa Iowa City, IA 52242
Yi Chu - Department of Internal Medicine Division of Cardiovascular Diseases Carver College of Medicine Cardiovascular Center University of Iowa Iowa City, IA 52242
Frank M Faraci - Department of Internal Medicine Division of Cardiovascular Diseases Carver College of Medicine Cardiovascular Center University of Iowa Iowa City, IA 52242
Resource Type: Journal article
Publication Details: Hypertension (Dallas, Tex. 1979), Vol.54(3), pp.619-624
DOI: 10.1161/HYPERTENSIONAHA.109.137158
PMID: 19620507
PMCID: PMC2861503
ISSN: 0194-911X
eISSN: 1524-4563
Language: English
Date published: 09/2009
Academic Unit: Cardiovascular Medicine; Neuroscience and Pharmacology; Internal Medicine
Record Identifier: 9984040306102771

Metrics

12 Record Views

143 Times Cited - Web of Science

See more details