Journal article
Endogenous secretion of IL-4 maintains growth and Thy-1 expression of a transformed B cell clone
The Journal of immunology (1950), Vol.150(2), pp.399-406
01/15/1993
DOI: 10.4049/jimmunol.150.2.399
PMID: 8093458
Abstract
The CD5+ B cell lymphoma clone, CH12.LX, endogenously produces IL-4. Blocking the binding of this IL-4 to its cellular receptor inhibited the continuous proliferation of CH12.LX. mAb specific for either IL-4 or the IL-4R profoundly and specifically inhibited the proliferation of CH12.LX cells in a concentration-dependent manner, within 4 h after the addition of mAb. The addition of exogenous rIL-4 alone to CH12.LX cells had no effect on either proliferation or antibody secretion. However, exogenous rIL-4 was able to counteract the effects of anti-IL-4 antibody. Treatment of CH12.LX cells with antisense RNA oligodeoxynucleotides to IL-4 also specifically inhibited cell proliferation and decreased the levels of IL-4 secreted into the culture supernatants by more than 50%, without effect on total RNA or protein synthesis. Effects of antisense IL-4 were also blocked by addition of exogenous IL-4. Control oligodeoxynucleotides of equal size and base composition had no effect, and IL-4 antisense oligodeoxynucleotides did not effect the growth of a B cell lymphoma clone which does not produce IL-4. Blocking the binding of endogenously produced IL-4 to CH12.LX cells did not change the levels of membrane IL-4R or CD5 molecules. However, the constitutive expression of Thy-1 by these B cells was markedly decreased, and anti-Thy-1 antibodies decreased proliferation and PMA-induced aggregation of CH12.LX cells. Autocrine secretion of IL-4 thus appears to be required both for the continuous proliferation of CH12.LX B cells, as well as their expression of Thy-1, which may function either as a homotypic adhesion molecule or a signal transduction molecule for these cells. These findings indicate that endogenously produced lymphokines may play a critical role in the maintenance of B cell hyperproliferative disorders.
Details
- Title: Subtitle
- Endogenous secretion of IL-4 maintains growth and Thy-1 expression of a transformed B cell clone
- Creators
- Steven W Louie - Department of Microbiology, University of Iowa, Iowa City 52242Luis M RamirezArthur M KriegCharles R MaliszewskiGail A Bishop
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.150(2), pp.399-406
- Publisher
- United States
- DOI
- 10.4049/jimmunol.150.2.399
- PMID
- 8093458
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Grant note
- AI28847 / NIAID NIH HHS DK252-95 / NIDDK NIH HHS
- Language
- English
- Date published
- 01/15/1993
- Academic Unit
- Microbiology and Immunology; President
- Record Identifier
- 9984001110502771
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