Journal article
Endothelial Superoxide Production Is Altered in Sheep Programmed by Early Gestation Dexamethasone Exposure
Neonatology (Basel, Switzerland), Vol.93(1), pp.19-27
12/2007
DOI: 10.1159/000105521
PMID: 17630494
Abstract
Background: Animal models have demonstrated that maternal undernutrition or early gestation glucocorticoid exposure induces endothelial dysfunction in the offspring. Objectives: We sought to determine whether early gestation dexamethasone (DEX) exposure is further associated with increased vascular superoxide anion production. Methods: DEX (0.28 mg/kg/day i.v. for 48 h) was administered to pregnant ewes at 27–28 days’ gestation (term 145 days). Tissues were harvested from DEX-exposed and control lambs at 125 days’ gestation (n = 6 for each group) and 4 months following delivery (n = 9 and 12, respectively). Results: By lucigenin-enhanced chemiluminescence, coronary and mesenteric arteries from DEX-exposed fetuses exhibited diminished basal superoxide production (both p < 0.01). Similarly, DEX-exposed carotid arteries from 4-month-old lambs had decreased superoxide production (p < 0.01) that localized to the endothelium by endothelial cell culture and dihydroethidium fluorescence. In contrast, DEX-exposed coronary arteries from the 4-month-old sheep had increased superoxide production (p < 0.05). Although early gestation DEX exposure did not alter lipid peroxidation, DEX exposure was associated with significantly increased renal and cerebral cortex aconitase activity (consistent with decreased protein oxidation). These changes occurred in the absence of alterations in renal cortex superoxide dismutase activity. Conclusions: We conclude that in a DEX-exposure model of fetal programming, endothelial superoxide production and protein oxidation are decreased in the mesenteric and carotid circulation. This contrasts with the postnatal coronary artery-specific increase in superoxide production.
Details
- Title: Subtitle
- Endothelial Superoxide Production Is Altered in Sheep Programmed by Early Gestation Dexamethasone Exposure
- Creators
- Robert D RoghairFrancis J Miller JrThomas D ScholzFred S LambJeffrey L Segar
- Resource Type
- Journal article
- Publication Details
- Neonatology (Basel, Switzerland), Vol.93(1), pp.19-27
- DOI
- 10.1159/000105521
- PMID
- 17630494
- ISSN
- 1661-7800
- eISSN
- 1661-7819
- Number of pages
- 9
- Language
- English
- Date published
- 12/2007
- Academic Unit
- Cardiology; Stead Family Department of Pediatrics; Fraternal Order of Eagles Diabetes Research Center; Child and Community Health; Neonatology
- Record Identifier
- 9984093209102771
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