Endothelium-dependent relaxation and L-arginine metabolism in genetic hypertension

L Lee; R C Webb

doi:10.1161/01.HYP.19.5.435

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Endothelium-dependent relaxation and L-arginine metabolism in genetic hypertension

Journal article

Open access

Peer reviewed

Endothelium-dependent relaxation and L-arginine metabolism in genetic hypertension

L Lee and R C Webb

Hypertension (Dallas, Tex. 1979), Vol.19(5), pp.435-441

05/1992

DOI: 10.1161/01.HYP.19.5.435

PMID: 1568761

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Abstract

This study characterizes the effects of L-arginine and NG-monomethyl L-arginine on dilator responsiveness of vascular tissue from Wistar-Kyoto rats and stroke-prone spontaneously hypertensive rats. Rings of abdominal aorta were suspended in tissue baths for measurement of isometric force. After contraction induced by phenylephrine, cumulative addition of acetylcholine, L-arginine, or A23187 to the muscle bath caused a similar relaxation of aortic rings in both animal groups. To test the hypothesis that arginine metabolism is altered in hypertension, aortic rings were incubated with NG-monomethyl L-arginine. NG-monomethyl L-arginine (10-300 microM) did not affect contractile responses to phenylephrine (10(-10) to 10(-4) M) in either animal group (EC50, 10(-7) M). Exposure of aortic rings to NG-monomethyl L-arginine resulted in a greater inhibition of relaxation response to acetylcholine (10(-10) to 10(-6) M) in hypertensive animals. NG-monomethyl L-arginine (300 microM) caused complete inhibition of relaxation to acetylcholine in the hypertension group. Incubation with L-arginine (10-100 microM) overcame the inhibition of acetylcholine-induced relaxation produced by NG-monomethyl L-arginine in both groups. Exposure of aortic ring segments to NG-monomethyl L-arginine attenuated relaxation responses to A23187 (10(-10) to 3 x 10(-6) M) in both groups. L-Arginine-induced reversal of the inhibitory effect of NG-monomethyl L-arginine on the relaxation responses to A23187 was similar between groups.

Animals

Arginine - analogs & derivatives

Arginine - metabolism

Arginine - pharmacology

Endothelium, Vascular - drug effects

Endothelium, Vascular - physiology

Female

Hypertension - genetics

Hypertension - metabolism

Hypertension - physiopathology

Male

omega-N-Methylarginine

Phenylephrine - pharmacology

Rats

Rats, Inbred SHR

Rats, Inbred WKY

Vasodilation - drug effects

Vasodilation - genetics

Details

Title: Subtitle: Endothelium-dependent relaxation and L-arginine metabolism in genetic hypertension
Creators: L Lee - University of Michigan
R C Webb - University of Michigan
Resource Type: Journal article
Publication Details: Hypertension (Dallas, Tex. 1979), Vol.19(5), pp.435-441
DOI: 10.1161/01.HYP.19.5.435
PMID: 1568761
NLM abbreviation: Hypertension
ISSN: 0194-911X
eISSN: 1524-4563
Grant note: HL-27020 / NHLBI NIH HHS
Language: English
Date published: 05/1992
Academic Unit: Cardiovascular Medicine; Internal Medicine
Record Identifier: 9984359855402771

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