Energetics of chloride secretion in canine tracheal epithelium: comparison of the metabolic cost of chloride transport with the metabolic cost of sodium transport

M. J WELSH; P KARP

doi:10.1172/JCI111410

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Energetics of chloride secretion in canine tracheal epithelium: comparison of the metabolic cost of chloride transport with the metabolic cost of sodium transport

Journal article

Open access

Peer reviewed

Energetics of chloride secretion in canine tracheal epithelium: comparison of the metabolic cost of chloride transport with the metabolic cost of sodium transport

M. J WELSH and P KARP

The Journal of clinical investigation, Vol.74(1), pp.262-268

1984

DOI: 10.1172/JCI111410

PMID: 6736250

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Abstract

Canine tracheal epithelium secretes Cl and absorbs Na; the energy for both is derived from the activity of the basolateral membrane Na-K-ATPase. These properties allowed us to examine the energetics of Cl secretion by directly comparing the metabolic cost of Cl transport with the metabolic cost of Na transport. We measured the change in short-circuit current and O2 consumption rate that was produced by a variety of maneuvers that alter Na and/or Cl transport rate. The experimental interventions included the use of the secretagogue epinephrine, the Cl transport inhibitor bumetanide, the Na transport inhibitor amiloride, the Na-K-ATPase inhibitor ouabain, and ion substitutions. The O2 consumption rates required for Na and Cl transport were compared in each individual tissue. The results indicate that the oxygen cost of Cl transport is significantly less than the oxygen cost of Na transport: two to four Cl ions are transported for the same metabolic cost that is required to transport one Na ion. These findings suggest that the basolateral membrane Na-dependent Cl entry step couples the entry of more than one Cl ion to each Na ion.

Fundamental and applied biological sciences. Psychology

Biological and medical sciences

Air breathing

Vertebrates: respiratory system

Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics

Details

Title: Subtitle: Energetics of chloride secretion in canine tracheal epithelium: comparison of the metabolic cost of chloride transport with the metabolic cost of sodium transport
Creators: M. J WELSH - Univ. Iowa, coll. medicine, Iowa City IA 52242, United States
P KARP - Univ. Iowa, coll. medicine, Iowa City IA 52242, United States
Resource Type: Journal article
Publication Details: The Journal of clinical investigation, Vol.74(1), pp.262-268
Publisher: American Society for Clinical Investigation
DOI: 10.1172/JCI111410
PMID: 6736250
ISSN: 0021-9738
eISSN: 1558-8238
Language: English
Date published: 1984
Academic Unit: Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Fraternal Order of Eagles Diabetes Research Center; Neurosurgery; Internal Medicine
Record Identifier: 9984020748402771

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