Energy efficient bead milling of microalgae: Effect of bead size on disintegration and release of proteins and carbohydrates

P. R. Postma; E. Suarez-Garcia; C. Safi; K. Yonathan; G. Olivieri; M. J. Barbosa; R. H. Wijffels; M. H. M. Eppink

doi:10.1016/j.biortech.2016.11.071

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Energy efficient bead milling of microalgae: Effect of bead size on disintegration and release of proteins and carbohydrates

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Energy efficient bead milling of microalgae: Effect of bead size on disintegration and release of proteins and carbohydrates

P. R. Postma, E. Suarez-Garcia, C. Safi, K. Yonathan, G. Olivieri, M. J. Barbosa, R. H. Wijffels and M. H. M. Eppink

Bioresource technology, Vol.224, pp.670-679

01/01/2017

DOI: 10.1016/j.biortech.2016.11.071

PMID: 27914784

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Abstract

The disintegration of three industry relevant algae (Chlorella vulgaris, Neochloris oleoabundans and Tetraselmis suecica) was studied in a lab scale bead mill at different bead sizes (0.3-1 mm). Cell disintegration, proteins and carbohydrates released into the water phase followed a first order kinetics. The process is selective towards proteins over carbohydrates during early stages of milling. In general, smaller beads led to higher kinetic rates, with a minimum specific energy consumption of <= 0.47 kWh kg(DW)(1) for 0.3 mm beads. After analysis of the stress parameters (stress number and stress intensity), it appears that optimal disintegration and energy usage for all strains occurs in the 0.3-0.4 mm range. During the course of bead milling, the native structure of the marker protein Rubisco was retained, confirming the mildness of the disruption process. (C) 2016 The Authors. Published by Elsevier Ltd.

Agricultural Engineering

Agriculture

Biotechnology & Applied Microbiology

Energy & Fuels

Life Sciences & Biomedicine

Science & Technology

Technology

Details

Title: Subtitle: Energy efficient bead milling of microalgae: Effect of bead size on disintegration and release of proteins and carbohydrates
Creators: P. R. Postma - Wageningen University & Research
E. Suarez-Garcia - Wageningen University & Research
C. Safi - Wageningen University & Research
K. Yonathan - Wageningen University & Research
G. Olivieri - Wageningen University & Research
M. J. Barbosa - Wageningen University & Research
R. H. Wijffels - Nord University
M. H. M. Eppink - Wageningen University & Research
Resource Type: Journal article
Publication Details: Bioresource technology, Vol.224, pp.670-679
DOI: 10.1016/j.biortech.2016.11.071
PMID: 27914784
NLM abbreviation: Bioresour Technol
ISSN: 0960-8524
eISSN: 1873-2976
Publisher: Elsevier
Number of pages: 10
Grant note: IPOP Biorefinery from Wageningen University and Research Centre (The Netherlands) 12635 / STW AlgaePro4U
Language: English
Date published: 01/01/2017
Academic Unit: International Programs; Spanish and Portuguese
Record Identifier: 9984397917202771

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