Engineering a well-ordered, functional protein-gold nanoparticle assembly

Jasmina C. Cheung-Lau; Dage Liu; Katherine W. Pulsipher; Weiren Liu; Ivan J. Dmochowski

doi:10.1016/j.jinorgbio.2013.10.003

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Engineering a well-ordered, functional protein-gold nanoparticle assembly

Journal article

Peer reviewed

Engineering a well-ordered, functional protein-gold nanoparticle assembly

Jasmina C. Cheung-Lau, Dage Liu, Katherine W. Pulsipher, Weiren Liu and Ivan J. Dmochowski

Journal of inorganic biochemistry, Vol.130(1), pp.59-68

01/01/2014

DOI: 10.1016/j.jinorgbio.2013.10.003

PMID: 24176920

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Abstract

The study of interactions between proteins and nanoparticles is important to advancing applications of nanoparticles in biology, medicine, and materials science. Here, we report the encapsulation of a 5-nm diameter gold nanoparticle (AuNP) by thermophilic ferritin (tF), achieved in nearly quantitative yield under mild conditions that preserved the secondary structure, ferroxidase activity, and thermal stability of the native, 4-helix bundle protein subunits. Chromatography-based assays determined that stable protein assembly around AuNPs occurred on long time scales (similar to 48 h) and was reversible. Apparent association constants were determined at 25 degrees C for equilibrated tF-BSPP-capped AuNP samples (K-A = (2.1 +/- 0.4) x 10(78) M-11) and compared favorably to salt-assembled tF samples (K-A = (2.2 +/- 0.5) x 10(66) M-11) at the same protein concentration (0.3 mg/mL). Finally, addition of gold ions and mild reducing agent to the tF-AuNP assembly produced 8-nm diameter AuNPs with surface plasmon resonance band unchanged at 520 nm, indicative of templating by the protein shell. (C) 2013 Elsevier Inc. All rights reserved.

Biochemistry & Molecular Biology

Chemistry

Chemistry, Inorganic & Nuclear

Life Sciences & Biomedicine

Physical Sciences

Science & Technology

Details

Title: Subtitle: Engineering a well-ordered, functional protein-gold nanoparticle assembly
Creators: Jasmina C. Cheung-Lau - University of Pennsylvania
Dage Liu - University of Pennsylvania
Katherine W. Pulsipher - University of Pennsylvania
Weiren Liu - University of Pennsylvania
Ivan J. Dmochowski - University of Pennsylvania
Resource Type: Journal article
Publication Details: Journal of inorganic biochemistry, Vol.130(1), pp.59-68
DOI: 10.1016/j.jinorgbio.2013.10.003
PMID: 24176920
NLM abbreviation: J Inorg Biochem
ISSN: 0162-0134
eISSN: 1873-3344
Publisher: Elsevier
Number of pages: 10
Grant note: 0548188; DMR-0520020 / NSF CHE; National Science Foundation (NSF)
Language: English
Date published: 01/01/2014
Academic Unit: Radiation Oncology
Record Identifier: 9985222825502771

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