Journal article
Enhanced Differentiation and Delivery of Mouse Retinal Progenitor Cells Using a Micropatterned Biodegradable Thin-Film Polycaprolactone Scaffold
Tissue engineering. Part A, Vol.21(7-8), pp.1247-1260
04/01/2015
DOI: 10.1089/ten.tea.2013.0720
PMCID: PMC4394889
PMID: 25517296
Abstract
The deterioration of retinal tissue in advanced stages of retinitis pigmentosa and age-related macular degeneration and the lack of signaling cues for laminar regeneration are significant challenges highlighting the need for a tissue engineering approach to retinal repair. In this study, we fabricated a biodegradable thin-film polycaprolactone (PCL) scaffold with varying surface topographies using microfabrication techniques. Mouse retinal progenitor cells (mRPCs) cultured on PCL scaffolds exhibited enhanced potential to differentiate toward a photoreceptor fate in comparison to mRPCs cultured on control substrates, suggesting that PCL scaffolds are promising as substrates to guide differentiation of mRPCs toward a photoreceptor fate in vitro before transplantation. When cocultured with the retinal explants of rhodopsin null mice, mRPC/PCL constructs showed increased mRPC integration rates compared to directly applied dissociated mRPCs. Moreover, these mRPC/PCL constructs could be delivered into the subretinal space of rhodopsin null mice with minimal disturbance of the host retina. Whether cocultured with retinal explants or transplanted into the subretinal space, newly integrated mRPCs localized to the outer nuclear layer and expressed appropriate markers of photoreceptor fate. Thus, the PCL scaffold provides a platform to guide differentiation and organized delivery of mRPCs as a practical strategy to repair damaged retina.
Details
- Title: Subtitle
- Enhanced Differentiation and Delivery of Mouse Retinal Progenitor Cells Using a Micropatterned Biodegradable Thin-Film Polycaprolactone Scaffold
- Creators
- Jing Yao - 2Department of Ophthalmology, Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School, Boston, MassachusettsChi Wan Ko - 4Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MassachusettsPetr Y Baranov - 2Department of Ophthalmology, Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School, Boston, MassachusettsCaio V Regatieri - 2Department of Ophthalmology, Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School, Boston, MassachusettsStephen Redenti - 5Department of Biological Sciences, Lehman College, City University of New York, Bronx, New YorkBudd A Tucker - 2Department of Ophthalmology, Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School, Boston, MassachusettsJason Mighty - 5Department of Biological Sciences, Lehman College, City University of New York, Bronx, New YorkSarah L Tao - 3The Charles Stark Draper Laboratory, Inc., Cambridge, MassachusettsMichael J Young - 2Department of Ophthalmology, Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts
- Resource Type
- Journal article
- Publication Details
- Tissue engineering. Part A, Vol.21(7-8), pp.1247-1260
- DOI
- 10.1089/ten.tea.2013.0720
- PMID
- 25517296
- PMCID
- PMC4394889
- NLM abbreviation
- Tissue Eng Part A
- ISSN
- 1937-3341
- eISSN
- 1937-335X
- Publisher
- Mary Ann Liebert, Inc
- Language
- English
- Date published
- 04/01/2015
- Academic Unit
- Ophthalmology and Visual Sciences
- Record Identifier
- 9983980086402771
Metrics
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