Journal article
Enhanced Surfactant Protein and Defensin mRNA Levels and Reduced Viral Replication during Parainfluenza Virus Type 3 Pneumonia in Neonatal Lambs
Clinical and diagnostic laboratory immunology, Vol.11(3), pp.599-607
05/2004
DOI: 10.1128/CDLI.11.3.599-607.2004
PMCID: PMC404576
PMID: 15138188
Abstract
Defensins and surfactant protein A (SP-A) and SP-D are antimicrobial components of the pulmonary innate immune system. The purpose of this study was to determine the extent to which parainfluenza type 3 virus infection in neonatal lambs alters expression of sheep beta-defensin 1 (SBD-1), SP-A, and SP-D, all of which are constitutively transcribed by respiratory epithelia. Parainfluenza type 3 viral antigen was detected by immunohistochemistry (IHC) in the bronchioles of all infected lambs 3 days postinoculation and at diminished levels 6 days postinoculation, but it was absent 17 days postinoculation. At all times postinoculation, lung homogenates from parainfluenza type 3 virus-inoculated animals had increased SBD-1, SP-A, and SP-D mRNA levels as detected by fluorogenic real-time reverse transcriptase PCR. Protein levels of SP-A in lung homogenates detected by quantitative-competitive enzyme-linked immunosorbent assay and protein antigen of SP-A detected by IHC were not altered. These studies demonstrate that parainfluenza type 3 virus infection results in enhanced expression of constitutively transcribed innate immune factors expressed by respiratory epithelia and that this increased expression occurs concurrently with decreased viral replication.
Details
- Title: Subtitle
- Enhanced Surfactant Protein and Defensin mRNA Levels and Reduced Viral Replication during Parainfluenza Virus Type 3 Pneumonia in Neonatal Lambs
- Creators
- Branka Grubor - Department of Veterinary PathologyJack M Gallup - Department of Veterinary PathologyDavid K Meyerholz - Department of Veterinary PathologyErika C Crouch - Department of Veterinary PathologyRichard B Evans - Department of Veterinary PathologyKim A Brogden - Department of Veterinary PathologyHoward D Lehmkuhl - Department of Veterinary PathologyMark R Ackermann - Department of Veterinary Pathology
- Resource Type
- Journal article
- Publication Details
- Clinical and diagnostic laboratory immunology, Vol.11(3), pp.599-607
- Publisher
- American Society for Microbiology
- DOI
- 10.1128/CDLI.11.3.599-607.2004
- PMID
- 15138188
- PMCID
- PMC404576
- ISSN
- 1071-412X
- eISSN
- 1098-6588
- Language
- English
- Date published
- 05/2004
- Academic Unit
- Pathology; Periodontics
- Record Identifier
- 9984083872102771
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