Journal article
Enhanced expression of ANGPTL2 in the microvascular lesions of diabetic glomerulopathy
Nephron. Experimental nephrology, Vol.105(4), pp.E117-E123
01/01/2007
DOI: 10.1159/000100493
PMID: 17347581
Abstract
Background: Diabetic nephropathy (DN) is one of the most important microvascular complications of diabetes mellitus. However, the underlying mechanisms remain unclear. We studied the expression characteristics of angiopoietin-like 2 (ANGPTL2), a novel DN-associated growth factor identified in our previous gene chip screening. Methods: Glomeruli were microdissected from renal biopsies from 24 patients with DN and 8 donor controls. The expression of ANGPTL2 was assessed by RT-PCR and immunohistochemistry, and then correlated with clinical and pathological indices of glomerular injury. Results: Consistent with the results of the gene chip experiment, abundant expression of ANGPTL2 was found more frequently in diabetic glomeruli as compared to donor controls (95 vs. 38%, chi(2) = 15.9, p < 0.01). ANGPTL2 mRNA upregulation was more prominent in glomeruli with less microaneurysm (22 vs. 66%, p < 0.05), inflammatory influx (6 vs. 50%, p < 0.05) or endothelial foam cell formation (11 vs. 53%, p < 0.05). Immunostaining revealed an upregulation of ANGPTL2 protein in hypertrophied diabetic glomeruli, mainly distributed in podocytes, which were supposed to be the origin of ANGPTL2. Conclusion: The upregulation of ANGPTL2 in diabetic glomerulopathy shows a close relationship to abnormal microvasculature and endothelial inflammation. ANGPTL2 may play an important role in the pathogenesis of diabetic glomerulopathy. Copyright (c) 2007 S. Karger AG, Basel.
Details
- Title: Subtitle
- Enhanced expression of ANGPTL2 in the microvascular lesions of diabetic glomerulopathy
- Creators
- Hua Sun - Nanjing UniversityJing-min ZhengShan ChenCai-hong ZengZhi-hong LiuLei-shi Li
- Resource Type
- Journal article
- Publication Details
- Nephron. Experimental nephrology, Vol.105(4), pp.E117-E123
- Publisher
- Karger
- DOI
- 10.1159/000100493
- PMID
- 17347581
- ISSN
- 1660-2129
- eISSN
- 1660-2129
- Number of pages
- 7
- Language
- English
- Date published
- 01/01/2007
- Academic Unit
- Nephrology, Dialysis and Transplantation; Stead Family Department of Pediatrics
- Record Identifier
- 9984384345602771
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