Journal article
Enhanced mast cell activation in mice deficient in the A2b adenosine receptor
The Journal of experimental medicine, Vol.204(1), pp.117-128
01/22/2007
DOI: 10.1084/jem.20061372
PMCID: PMC2118561
PMID: 17200408
Abstract
Antigen-mediated cross-linking of IgE bound to mast cells via the high affinity receptor for IgE triggers a signaling cascade that results in the release of intracellular calcium stores, followed by an influx of extracellular calcium. The collective increase in intracellular calcium is critical to the release of the granular contents of the mast cell, which include the mediators of acute anaphylaxis. We show that the sensitivity of the mast cell to antigen-mediated degranulation through this pathway can be dramatically influenced by the A2b adenosine receptor. Loss of this Gs-coupled receptor on mouse bone marrow–derived mast cells results in decreased basal levels of cyclic AMP and an excessive influx of extracellular calcium through store-operated calcium channels following antigen activation. Mice lacking the A2b receptor display increased sensitivity to IgE-mediated anaphylaxis. Collectively, these findings show that the A2b adenosine receptor functions as a critical regulator of signaling pathways within the mast cell, which act in concert to limit the magnitude of mast cell responsiveness when antigen is encountered.
Details
- Title: Subtitle
- Enhanced mast cell activation in mice deficient in the A2b adenosine receptor
- Creators
- Xiaoyang Hua - Department of Medicine, Division of Pulmonary and Critical Care MedicineMartina Kovarova - Department of Medicine, Division of Pulmonary and Critical Care MedicineKelly D. Chason - Department of Medicine, Division of Pulmonary and Critical Care MedicineMyTrang Nguyen - University of North Carolina at Chapel HillBeverly H. Koller - Department of Medicine, Division of Pulmonary and Critical Care MedicineStephen L. Tilley - Department of Medicine, Division of Pulmonary and Critical Care Medicine
- Resource Type
- Journal article
- Publication Details
- The Journal of experimental medicine, Vol.204(1), pp.117-128
- DOI
- 10.1084/jem.20061372
- PMID
- 17200408
- PMCID
- PMC2118561
- NLM abbreviation
- J Exp Med
- ISSN
- 0022-1007
- eISSN
- 1540-9538
- Language
- English
- Date published
- 01/22/2007
- Academic Unit
- Otolaryngology
- Record Identifier
- 9984311438402771
Metrics
9 Record Views