Journal article
Enhancement of MicroRNA-200c on Osteogenic Differentiation and Bone Regeneration by Targeting Sox2-Mediated Wnt Signaling and Klf4
Human gene therapy, Vol.30(11), pp.1405-1418
11/01/2019
DOI: 10.1089/hum.2019.019
PMCID: PMC6854517
PMID: 31288577
Abstract
MicroRNA (miR)-200c functions in antitumorigenesis and mediates inflammation and osteogenic differentiation. In this study, we discovered that
miR-200c
was upregulated in human bone marrow mesenchymal stromal cells (hBMSCs) during osteogenic differentiation. Inhibition of endogenous
miR-200c
resulted in downregulated osteogenic differentiation of hBMSCs and reduced bone volume in the maxilla and mandible of a transgenic mouse model. Overexpression of
miR-200c
by transfection of naked plasmid DNA (pDNA) encoding
miR-200c
significantly promoted the biomarkers of osteogenic differentiation in hBMSCs, including alkaline phosphatase, Runt-related transcription factor 2, osteocalcin, and mineral deposition. The pDNA encoding
miR-200c
also significantly enhanced bone formation and regeneration in calvarial defects of rat models. In addition,
miR-200c
overexpression was shown to downregulate SRY (sex determining region Y)-box 2 (
Sox2
) and Kruppel-like factor 4 by directly targeting
3′
-untranslated regions and upregulate the activity of Wnt signaling inhibited by
Sox2
. These results strongly indicated that
miR-200c
may serve as a unique osteoinductive agent applied for bone healing and regeneration.
Details
- Title: Subtitle
- Enhancement of MicroRNA-200c on Osteogenic Differentiation and Bone Regeneration by Targeting Sox2-Mediated Wnt Signaling and Klf4
- Creators
- Adil Akkouch - University of IowaSteven Eliason - University of IowaMason E. Sweat - University of IowaMiguel Romero-Bustillos - University of IowaMin Zhu - University of IowaFang Qian - University of IowaBrad A. Amendt - University of IowaLiu Hong - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Human gene therapy, Vol.30(11), pp.1405-1418
- DOI
- 10.1089/hum.2019.019
- PMID
- 31288577
- PMCID
- PMC6854517
- NLM abbreviation
- Hum Gene Ther
- ISSN
- 1043-0342
- eISSN
- 1557-7422
- Publisher
- Mary Ann Liebert, Inc., publishers
- Language
- English
- Date published
- 11/01/2019
- Academic Unit
- Preventive and Community Dentistry; Roy J. Carver Department of Biomedical Engineering; Orthodontics; Anatomy and Cell Biology; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984284450502771
Metrics
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