Journal article
Enhancement of adherence and growth of Chlamydia trachomatis by estrogen treatment of HeLa cells
Infection and immunity, Vol.53(3), pp.646-650
1986
DOI: 10.1128/IAI.53.3.646-650.1986
PMCID: PMC260842
PMID: 3744558
Abstract
Treatment of HeLa 229 cultures with 17-beta-estradiol or with diethylstilbestrol, a synthetic estrogen analog, prior to infection with Chlamydia trachomatis UW31 (serovar K) or LGV440 (serovar L1) led to a 50 to 60% enhancement of chlamydial inclusion formation. After infection, the presence of estrogen was required for the enhancement. The optimal concentration of estrogen required was 10(-10) M. At least 18 h of preinfection treatment plus 12 h of postinfection treatment was necessary. The adherence of purified radioactive elementary bodies of C. trachomatis to estrogen-treated HeLa cells was stimulated in an estrogen dose- and exposure-dependent manner. The requirements for both pre- and postinfection exposure to the hormone suggest that alterations in the cell membrane as well as in the metabolic capacity of the host cells is required for intracellular chlamydial development. Cycloheximide did not prevent estrogen enhancement of chlamydial adherence or subsequent intracellular development of inclusions.
Details
- Title: Subtitle
- Enhancement of adherence and growth of Chlamydia trachomatis by estrogen treatment of HeLa cells
- Creators
- Subir K Bose - St. Louis univ., school medicine, St. Louis MO 63104, United StatesP. C GOSWAMI - St. Louis univ., school medicine, St. Louis MO 63104, United States
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.53(3), pp.646-650
- Publisher
- American Society for Microbiology; Washington, DC
- DOI
- 10.1128/IAI.53.3.646-650.1986
- PMID
- 3744558
- PMCID
- PMC260842
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Language
- English
- Date published
- 1986
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984046923202771
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