Enhancement of murine coronavirus replication by severe acute respiratory syndrome coronavirus protein 6 requires the N-terminal hydrophobic region but not C-terminal sorting motifs

Jason Netland; Debra Ferraro; Lecia Pewe; Heidi Olivares; Thomas Gallagher; Stanley Perlman

doi:10.1128/JVI.01308-07

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Enhancement of murine coronavirus replication by severe acute respiratory syndrome coronavirus protein 6 requires the N-terminal hydrophobic region but not C-terminal sorting motifs

Journal article

Open access

Peer reviewed

Enhancement of murine coronavirus replication by severe acute respiratory syndrome coronavirus protein 6 requires the N-terminal hydrophobic region but not C-terminal sorting motifs

Jason Netland, Debra Ferraro, Lecia Pewe, Heidi Olivares, Thomas Gallagher and Stanley Perlman

Journal of virology, Vol.81(20), pp.11520-11525

10/2007

DOI: 10.1128/JVI.01308-07

PMCID: PMC2045524

PMID: 17670827

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Abstract

Severe acute respiratory syndrome coronavirus encodes several accessory proteins of unknown function. We previously showed that one such protein, encoded by ORF6, enhanced the growth of mouse hepatitis virus in tissue culture cells and in mice. Protein 6 consists of an N-terminal hydrophobic peptide and a C-terminal region containing intracellular protein sorting motifs. Herein, we show that mutation of the hydrophobic region but not the sorting motifs affected the ability of protein 6 to enhance virus growth. Collectively, these results support the notion that the 6 protein interacts with membrane-bound viral replication or assembly machinery to directly enhance virus replication and virulence in animals.

Coronavirus Infections

Animals

Virus Replication

Viral Proteins - chemistry

Viral Proteins - physiology

Hydrophobic and Hydrophilic Interactions

Viral Proteins - genetics

Mice

Mutation

SARS Virus - chemistry

Amino Acid Motifs

Details

Title: Subtitle: Enhancement of murine coronavirus replication by severe acute respiratory syndrome coronavirus protein 6 requires the N-terminal hydrophobic region but not C-terminal sorting motifs
Creators: Jason Netland - Department of Microbiology, University of Iowa, BSB 3-712, Iowa City, IA 52242, USA
Debra Ferraro
Lecia Pewe
Heidi Olivares
Thomas Gallagher
Stanley Perlman
Resource Type: Journal article
Publication Details: Journal of virology, Vol.81(20), pp.11520-11525
DOI: 10.1128/JVI.01308-07
PMID: 17670827
PMCID: PMC2045524
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Publisher: United States
Grant note: T32 HL07638 / NHLBI NIH HHS P01 AI060699 / NIAID NIH HHS T32 HL007638 / NHLBI NIH HHS T32 AI007533 / NIAID NIH HHS
Language: English
Date published: 10/2007
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777471402771

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