Journal article
Enhancement of peritoneal leukocyte function by granulocyte colony-stimulating factor in rats with abdominal sepsis
Critical care medicine, Vol.26(2), pp.315-321
02/1998
DOI: 10.1097/00003246-199802000-00035
PMID: 9468171
Abstract
OBJECTIVETo investigate the therapeutic effects of granulocyte colony-stimulating factor (G-CSF) on the functional activities of circulating and peritoneal neutrophils during intra-abdominal sepsis.
DESIGNPlacebo, controlled study, using a rat model of intra-abdominal sepsis.
SETTINGAnimal research facility.
SUBJECTSMale specific pathogen-free Sprague-Dawley rats.
INTERVENTIONSAbdominal sepsis was produced in rats by cecal ligation and puncture. The control animals received a sham operation. G-CSF (subcutaneous injection at 50 [micro sign]g/kg) or vehicle (100 [micro sign]L of 5% dextrose) treatment was initiated at 1 hr after cecal ligation and puncture or sham operation and repeated at 12-hr intervals thereafter.
MEASUREMENTS AND MAIN RESULTSSix hours after cecal ligation and puncture, CD11b/c and CD18 expression on circulating neutrophils was significantly up-regulated when compared with those in the sham operated control animals. Peritoneal neutrophils exhibited a further up-regulation of these adhesion molecules than did the circulating neutrophils. A sustained up-regulation of CD11b/c and CD18 was found in peritoneal neutrophils even at 24 hrs after cecal ligation and puncture. G-CSF treatment increased CD11b/c expression on circulating neutrophils in 6-hr sham-operated rats, but did not further up-regulate CD11b/c or CD18 expression on circulating or peritoneal neutrophils in cecal ligation and puncture rats. Phagocytic activities of circulating neutrophils assessed by uptake of fluorescent latex microspheres were lower in 24-hr cecal ligation and puncture rats when compared with the sham-operated controls. G-CSF treatment prevented this inhibition. Furthermore, G-CSF enhanced the phagocytic activities of peritoneal neutrophils in both 6- and 24-hr cecal ligation and puncture rats when compared with those of the vehicle-treated animals. Spontaneous hydrogen peroxide generation by circulating neutrophils was increased in 6-hr cecal ligation and puncture rats, but not in 24-hr cecal ligation and puncture rats. Peritoneal neutrophils exhibited an inhibition of phorbol myristate acetate-stimulated hydrogen peroxide generation. G-CSF treatment did not up-regulate neutrophil hydrogen peroxide generation.
CONCLUSIONSCirculating and peritoneal neutrophils exhibit marked polymorphism in their functional activities during the host response to abdominal sepsis. G-CSF treatment significantly enhanced the phagocytic function of both circulating and peritoneal neutrophils which may be one mechanism underlying its protective effect in abdominal sepsis. (Crit Care Med 1998; 26:315-321)
Details
- Title: Subtitle
- Enhancement of peritoneal leukocyte function by granulocyte colony-stimulating factor in rats with abdominal sepsis
- Creators
- Ping Zhang - From the Department of Medicine, Section of Pulmonary and Critical Care Medicine (Drs. Zhang, Summer, and Nelson); and the Department of Physiology (Dr. Bagby and Mr. Stoltz), Louisiana State University Medical Center, New Orleans, LA. Supported, in part, by grant AA-09803 from the National Institutes of HealthGregory J BagbyDavid A StoltzWarren R SummerSteve Nelson
- Resource Type
- Journal article
- Publication Details
- Critical care medicine, Vol.26(2), pp.315-321
- Publisher
- Williams & Wilkins
- DOI
- 10.1097/00003246-199802000-00035
- PMID
- 9468171
- ISSN
- 0090-3493
- eISSN
- 1530-0293
- Language
- English
- Date published
- 02/1998
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Internal Medicine
- Record Identifier
- 9984025572302771
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