Enhancer action in trans is permitted throughout the Drosophila genome

Ji-Long Chen; Kathryn L Huisinga; Michaela M Viering; Sharon A Ou; C.-ting Wu; Pamela K Geyer

doi:10.1073/pnas.062447999

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Enhancer action in trans is permitted throughout the Drosophila genome

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Enhancer action in trans is permitted throughout the Drosophila genome

Ji-Long Chen, Kathryn L Huisinga, Michaela M Viering, Sharon A Ou, C.-ting Wu and Pamela K Geyer

Proceedings of the National Academy of Sciences - PNAS, Vol.99(6), pp.3723-3728

03/19/2002

DOI: 10.1073/pnas.062447999

PMCID: PMC122591

PMID: 11904429

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Abstract

Interactions between paired homologous genes can lead to changes in gene expression. Such trans-regulatory effects exemplify transvection and are displayed by many genes in Drosophila , in which homologous chromosomes are paired somatically. Transvection involving the yellow cuticle pigmentation gene can occur by at least two mechanisms, one involving the trans-action of enhancers on a paired promoter and a second involving pairing-mediated bypass of a chromatin insulator. A system was developed to evaluate whether the action of the yellow enhancers in trans could be reconstituted outside of the natural near telomeric location of the yellow gene. To this end, transgenic flies were generated that carried a yellow gene modified by the inclusion of strategically placed recognition sites for the Cre and FLP recombinases. Independent action of the recombinases produced a pair of derivative alleles, one enhancerless and the other promoterless, at each transgene location. Transvection between the derivatives was assessed by the degree of interallelic complementation. Complementation was observed at all eight sites tested. These studies demonstrate that yellow transvection can occur at multiple genomic locations and indicate that the Drosophila genome generally is permissive to enhancer action in trans.

Biological Sciences

Details

Title: Subtitle: Enhancer action in trans is permitted throughout the Drosophila genome
Creators: Ji-Long Chen - Department of Biochemistry, University of Iowa, Iowa City, IA 52242; and
Kathryn L Huisinga - Department of Biochemistry, University of Iowa, Iowa City, IA 52242; and
Michaela M Viering - Department of Biochemistry, University of Iowa, Iowa City, IA 52242; and
Sharon A Ou - Department of Biochemistry, University of Iowa, Iowa City, IA 52242; and
C.-ting Wu - Department of Biochemistry, University of Iowa, Iowa City, IA 52242; and
Pamela K Geyer - Department of Biochemistry, University of Iowa, Iowa City, IA 52242; and
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.99(6), pp.3723-3728
DOI: 10.1073/pnas.062447999
PMID: 11904429
PMCID: PMC122591
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Language: English
Date published: 03/19/2002
Academic Unit: Obstetrics and Gynecology; Biochemistry and Molecular Biology
Record Identifier: 9984024532802771

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