Journal article
Enrichment of NPC1-deficient cells with the lipid LBPA stimulates autophagy, improves lysosomal function, and reduces cholesterol storage
The Journal of biological chemistry, Vol.297(1), pp.100813-100813
07/2021
DOI: 10.1016/j.jbc.2021.100813
PMCID: PMC8294588
PMID: 34023384
Abstract
Niemann–Pick C (NPC) is an autosomal recessive disorder characterized by mutations in the NPC1 or NPC2 genes encoding endolysosomal lipid transport proteins, leading to cholesterol accumulation and autophagy dysfunction. We have previously shown that enrichment of NPC1-deficient cells with the anionic lipid lysobisphosphatidic acid (LBPA; also called bis(monoacylglycerol)phosphate) via treatment with its precursor phosphatidylglycerol (PG) results in a dramatic decrease in cholesterol storage. However, the mechanisms underlying this reduction are unknown. In the present study, we showed using biochemical and imaging approaches in both NPC1-deficient cellular models and an NPC1 mouse model that PG incubation/LBPA enrichment significantly improved the compromised autophagic flux associated with NPC1 disease, providing a route for NPC1-independent endolysosomal cholesterol mobilization. PG/LBPA enrichment specifically enhanced the late stages of autophagy, and effects were mediated by activation of the lysosomal enzyme acid sphingomyelinase. PG incubation also led to robust and specific increases in LBPA species with polyunsaturated acyl chains, potentially increasing the propensity for membrane fusion events, which are critical for late-stage autophagy progression. Finally, we demonstrated that PG/LBPA treatment efficiently cleared cholesterol and toxic protein aggregates in Purkinje neurons of the NPC1I1061T mouse model. Collectively, these findings provide a mechanistic basis supporting cellular LBPA as a potential new target for therapeutic intervention in NPC disease.
Details
- Title: Subtitle
- Enrichment of NPC1-deficient cells with the lipid LBPA stimulates autophagy, improves lysosomal function, and reduces cholesterol storage
- Creators
- Olga Ilnytska - Rutgers, The State University of New JerseyKimberly Lai - Rutgers, The State University of New JerseyKirill Gorshkov - National Center for Advancing Translational SciencesMark L. Schultz - University of Michigan Medical SchoolBruce Nguyen Tran - National Center for Advancing Translational SciencesMaciej Jeziorek - Rutgers University–NewarkThaddeus J. Kunkel - University of MichiganRuth D. Azaria - University of MichiganHayley S. McLoughlin - University of MichiganMiriam Waghalter - Rutgers, The State University of New JerseyYang Xu - New York UniversityMichael Schlame - New York UniversityNihal Altan-Bonnet - National Heart Lung and Blood InstituteWei Zheng - National Center for Advancing Translational SciencesAndrew P. Lieberman - University of MichiganRadek Dobrowolski - Rutgers, The State University of New JerseyJudith Storch - Rutgers, The State University of New Jersey
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.297(1), pp.100813-100813
- DOI
- 10.1016/j.jbc.2021.100813
- PMID
- 34023384
- PMCID
- PMC8294588
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Publisher
- Elsevier Inc
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health; DOI: 10.13039/100000968, name: American Heart Association Inc; DOI: 10.13039/100001790, name: Ara Parseghian Medical Research Foundation
- Language
- English
- Date published
- 07/2021
- Academic Unit
- Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Medical Genetics and Genomics
- Record Identifier
- 9984366289602771
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