Entamoeba histolytica modulates a complex repertoire of novel genes in response to oxidative and nitrosative stresses: implications for amebic pathogenesis

Joao B. Vicente; Gretchen M. Ehrenkaufer; Ligia M. Saraiva; Miguel Teixeira; Upinder Singh

doi:10.1111/j.1462-5822.2008.01236.x

Back

Entamoeba histolytica modulates a complex repertoire of novel genes in response to oxidative and nitrosative stresses: implications for amebic pathogenesis

Journal article

Open access

Peer reviewed

Entamoeba histolytica modulates a complex repertoire of novel genes in response to oxidative and nitrosative stresses: implications for amebic pathogenesis

Joao B. Vicente, Gretchen M. Ehrenkaufer, Ligia M. Saraiva, Miguel Teixeira and Upinder Singh

Cellular microbiology, Vol.11(1), pp.51-69

01/01/2009

DOI: 10.1111/j.1462-5822.2008.01236.x

PMID: 18778413

Files and links (1)

url

https://doi.org/10.1111/j.1462-5822.2008.01236.xView

Published (Version of record) Open Access

Abstract

Upon host infection, the protozoan parasite Entamoeba histolytica is confronted with reactive oxygen and nitrogen species and must survive these stresses in order to cause invasive disease. We analysed the parasite's response to oxidative and nitrosative stresses, probing the transcriptional changes of trophozoites of a pathogenic strain after a 60 min exposure to H2O2 (1 mM) or a NO donor (dipropylenetriamine-NONOate, 200 mu M), using whole-genome DNA microarrays. Genes encoding reactive oxygen and nitrogen species detoxification enzymes had high transcriptional levels under basal conditions and upon exposure to both stresses. On a whole-genome level, there was significant modulation of gene expression by H2O2 (286 genes regulated) and dipropylenetriamine-NONOate (1036 genes regulated) with a significant overlap of genes modulated under both conditions (164 genes). A number of transcriptionally regulated genes were in signalling/regulatory and repair/metabolic pathways. However, the majority of genes with altered transcription encode unknown proteins, suggesting as yet unraveled response pathways in E. histolytica. Trophozoites of a non-pathogenic E. histolytica strain had a significantly muted transcriptional response to H2O2 compared with the pathogenic strain, hinting that differential response to oxidative stress may be one factor that contributes to the pathogenic potential of E. histolytica.

Cell Biology

Life Sciences & Biomedicine

Microbiology

Science & Technology

Details

Title: Subtitle: Entamoeba histolytica modulates a complex repertoire of novel genes in response to oxidative and nitrosative stresses: implications for amebic pathogenesis
Creators: Joao B. Vicente - Stanford Medicine
Gretchen M. Ehrenkaufer - Stanford Medicine
Ligia M. Saraiva - Universidade Nova de Lisboa
Miguel Teixeira - Universidade Nova de Lisboa
Upinder Singh - Stanford Medicine
Resource Type: Journal article
Publication Details: Cellular microbiology, Vol.11(1), pp.51-69
DOI: 10.1111/j.1462-5822.2008.01236.x
PMID: 18778413
NLM abbreviation: Cell Microbiol
ISSN: 1462-5814
eISSN: 1462-5822
Publisher: Wiley
Number of pages: 19
Grant note: AI-053724 / NIAID; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) SFRH/BPD/26895/2006; POCI/SAU-IMI/56088/2004; PTDC/BIAPRO/67267/2006 / FCT; Fundacao para a Ciencia e a Tecnologia (FCT) AI-068899; AI-069382 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA SFRH/BPD/26895/2006; POCI/SAU-IMI/56088/2004 / Fundação para a Ciência e a Tecnologia; Fundacao para a Ciencia e a Tecnologia (FCT)
Language: English
Date published: 01/01/2009
Academic Unit: Internal Medicine
Record Identifier: 9985244564902771

Metrics

1 Record Views

87 Times Cited - Web of Science