Enteral Exclusion Increases Map Kinase Activation and Cytokine Production in a Model of Gallstone Pancreatitis

Isaac Samuel; Linda Tephly; Deborah E Williard; A. Brent Carter

doi:10.1159/000114850

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Enteral Exclusion Increases Map Kinase Activation and Cytokine Production in a Model of Gallstone Pancreatitis

Journal article

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Enteral Exclusion Increases Map Kinase Activation and Cytokine Production in a Model of Gallstone Pancreatitis

Isaac Samuel, Linda Tephly, Deborah E Williard and A. Brent Carter

Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], Vol.8(1), pp.6-14

04/2008

DOI: 10.1159/000114850

PMCID: PMC2829292

PMID: 18235211

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https://europepmc.org/articles/pmc2829292View

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Abstract

Background: We have previously demonstrated that enteral exclusion augments pancreatic p38 mitogen-activated protein (MAP) kinase activation and tumor necrosis factor-alpha (TNF-alpha) production after bile-pancreatic duct ligation in rats. Methods: In the present study, we evaluated c-Jun NH(2)-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) activation, and cytokine production, in pancreata of duct-ligated rats with and without duodenal bile-pancreatic juice replacement from a donor rat. We hypothesized that enteral exclusion of bile-pancreatic juice activates stress kinases and induces cytokine production in ligation-induced acute pancreatitis. Results: Increased JNK and ERK activation after ligation are inhibited by bile-pancreatic juice replacement. Increases in pancreatic production of IL-1beta and IL-12 after ligation are significantly subdued by replacement. In additional in vitro studies, we show that cholecystokinin- or TNF-alpha-stimulated nuclear transcription factor kappa-B activation in AR42J cells is inhibited by dominant negative ERK2. Conclusions: Our novel findings using our Donor Rat Model indicate that bile-pancreatic juice exclusion induces MAP kinase activation and exacerbates cell stress and inflammation in this experimental model of gallstone pancreatitis. and IAP.

c-Jun NH2-terminal kinase

Acute pancreatitis

Rapid Communication

Cytokine

Extracellular signal-regulated kinase

Stress-activated protein kinase

Cholecystokinin

Acinar cell

Donor Rat Model

Details

Title: Subtitle: Enteral Exclusion Increases Map Kinase Activation and Cytokine Production in a Model of Gallstone Pancreatitis
Creators: Isaac Samuel - Departments of Surgery, Iowa City, Iowa, USA
Linda Tephly - Internal Medicine, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa, USA
Deborah E Williard - Departments of Surgery, Iowa City, Iowa, USA
A. Brent Carter - Internal Medicine, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa, USA
Resource Type: Journal article
Publication Details: Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], Vol.8(1), pp.6-14
DOI: 10.1159/000114850
PMID: 18235211
PMCID: PMC2829292
NLM abbreviation: Pancreatology
ISSN: 1424-3903
eISSN: 1424-3911
Publisher: S. Karger AG
Language: English
Date published: 04/2008
Academic Unit: IIHR--Hydroscience and Engineering; Surgery; Radiation Oncology
Record Identifier: 9984051720202771

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