Journal article
Enterococcus faecalis Overcomes Foreign Body-Mediated Inflammation To Establish Urinary Tract Infections
Infection and immunity, Vol.81(1), pp.329-339
01/2013
DOI: 10.1128/IAI.00856-12
PMCID: PMC3536162
PMID: 23132492
Abstract
Urinary catheterization elicits major histological and immunological changes that render the bladder susceptible to microbial invasion, colonization, and dissemination. However, it is not understood how catheters induce these changes, how these changes act to promote infection, or whether they may have any protective benefit. In the present study, we examined how catheter-associated inflammation impacts infection by
Enterococcus faecalis
, a leading cause of catheter-associated urinary tract infection (CAUTI), a source of significant societal and clinical challenges. Using a recently optimized murine model of foreign body-associated UTI, we found that the implanted catheter itself was the primary inducer of inflammation. In the absence of the silicone tubing implant,
E. faecalis
induced only minimal inflammation and was rapidly cleared from the bladder. The catheter-induced inflammation was only minimally altered by subsequent enterococcal infection and was not suppressed by inhibitors of the neurogenic pathway and only partially by dexamethasone. Despite the robust inflammatory response induced by urinary implantation,
E. faecalis
produced biofilm and high bladder titers in these animals. Induction of inflammation in the absence of an implanted catheter failed to promote infection, suggesting that the presence of the catheter itself is essential for
E. faecalis
persistence in the bladder. Immunosuppression prior to urinary catheterization enhanced
E. faecalis
colonization, suggesting that implant-mediated inflammation contributes to the control of enterococcal infection. Thus, this study underscores the need for novel strategies against CAUTIs that seek to reduce the deleterious effects of implant-mediated inflammation on bladder homeostasis while maintaining an active immune response that effectively limits bacterial invaders.
Details
- Title: Subtitle
- Enterococcus faecalis Overcomes Foreign Body-Mediated Inflammation To Establish Urinary Tract Infections
- Creators
- Pascale S Guiton - Department of Molecular Microbiology and Microbial PathogenesisThomas J Hannan - Department of Pathology and Immunology, Washington University in St. Louis, Saint Louis, Missouri, USABradley Ford - Department of Molecular Microbiology and Microbial PathogenesisMichael G Caparon - Department of Molecular Microbiology and Microbial PathogenesisScott J Hultgren - Department of Molecular Microbiology and Microbial Pathogenesis
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.81(1), pp.329-339
- Publisher
- American Society for Microbiology; 1752 N St., N.W., Washington, DC
- DOI
- 10.1128/IAI.00856-12
- PMID
- 23132492
- PMCID
- PMC3536162
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Language
- English
- Date published
- 01/2013
- Academic Unit
- Pathology
- Record Identifier
- 9984047983302771
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