Journal article
Enumeration of an extremely high particle-to-PFU ratio for Varicella-zoster virus
Journal of virology, Vol.83(13), pp.6917-6921
07/2009
DOI: 10.1128/JVI.00081-09
PMCID: PMC2698559
PMID: 19369328
Abstract
Varicella-zoster virus (VZV) is renowned for its low titers. Yet investigations to explore the low infectivity are hampered by the fact that the VZV particle-to-PFU ratio has never been determined with precision. Herein, we accomplish that task by applying newer imaging technology. More than 300 images were taken of VZV-infected cells on 4 different samples at high magnification. We enumerated the total number of viral particles within 25 cm(2) of the infected monolayer at 415 million. Based on these numbers, the VZV particle:PFU ratio was approximately 40,000:1 for a cell-free inoculum.
Details
- Title: Subtitle
- Enumeration of an extremely high particle-to-PFU ratio for Varicella-zoster virus
- Creators
- John E Carpenter - Central Microscopy Research Facility, University of Iowa, Iowa City, Iowa 52242, USAErnesto P HendersonCharles Grose
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.83(13), pp.6917-6921
- DOI
- 10.1128/JVI.00081-09
- PMID
- 19369328
- PMCID
- PMC2698559
- NLM abbreviation
- J Virol
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Grant note
- 58939 / PHS HHS AI22795 / NIAID NIH HHS R01 AI022795 / NIAID NIH HHS
- Language
- English
- Date published
- 07/2009
- Academic Unit
- Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093224402771
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