Epidermal cells accelerate the restoration of the blood flow in diabetic ischemic limbs

Chunhua Jiao; Sarah Bronner; Keri L N Mercer; Don D Sheriff; Gina C Schatteman; Martine Dunnwald

doi:10.1242/jcs.00926

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Epidermal cells accelerate the restoration of the blood flow in diabetic ischemic limbs

Journal article

Open access

Peer reviewed

Epidermal cells accelerate the restoration of the blood flow in diabetic ischemic limbs

Chunhua Jiao, Sarah Bronner, Keri L N Mercer, Don D Sheriff, Gina C Schatteman and Martine Dunnwald

Journal of cell science, Vol.117(Pt 7), pp.1055-1063

03/01/2004

DOI: 10.1242/jcs.00926

PMID: 14970256

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Abstract

Epidermal progenitor cells (EpPCs) were long thought to be unipotent, giving rise only to other keratinocytes but recent studies question this assumption. Here, we investigated whether mouse EpPCs can adopt other antigenic and functional phenotypes. To test this, we injected freshly isolated and cultured EpPCs and transient amplifying cells into diabetic and non-diabetic mouse ischemic hindlimb and followed the cells' fate and the recovery of the ischemic limb blood flow over time. Both freshly isolated and cultured EpPCs and transient amplifying cells were incorporated into the vasculature of the ischemic limb 2 and 5 weeks post-injection, and some expressed endothelial cell but not keratinocyte antigens. Additionally, in the non-diabetic animals, first transient amplifying cells and then EpPCs accelerated the restoration of the blood flow. By contrast, in diabetic animals, only injected EpPCs or unsorted epidermal cells accelerated the restoration of the blood flow. These data indicate that epidermal cells can adopt non-skin phenotypes and functions, and that this apparent pluripotency is not lost by differentiation of EpPCs into transient amplifying cells. They also suggest that epidermal cell therapy might be of therapeutic value in the treatment of diabetic ischemia. Finally, because epidermal cells are readily accessible and expandable, they appear to be ideally suited for use as a non-viral gene delivery therapy.

Mice, Inbred C57BL

Neovascularization, Pathologic

Ischemia - therapy

Keratinocytes - cytology

Ischemia - physiopathology

Regional Blood Flow

Diabetes Mellitus, Experimental - therapy

Stem Cell Transplantation

Phenotype

Animals

Hindlimb - blood supply

Diabetes Mellitus, Experimental - pathology

Cell Differentiation

Keratinocytes - transplantation

Mice

Muscle, Skeletal - pathology

Ischemia - pathology

Diabetes Mellitus, Experimental - physiopathology

Epidermis - cytology

Muscle, Skeletal - blood supply

Details

Title: Subtitle: Epidermal cells accelerate the restoration of the blood flow in diabetic ischemic limbs
Creators: Chunhua Jiao - Department of Exercise Science, The University of Iowa, Iowa City, IA 52242, USA
Sarah Bronner
Keri L N Mercer
Don D Sheriff
Gina C Schatteman
Martine Dunnwald
Resource Type: Journal article
Publication Details: Journal of cell science, Vol.117(Pt 7), pp.1055-1063
DOI: 10.1242/jcs.00926
PMID: 14970256
NLM abbreviation: J Cell Sci
ISSN: 0021-9533
eISSN: 1477-9137
Publisher: England
Grant note: DK59223 / NIDDK NIH HHS DK55965 / NIDDK NIH HHS P30 DK54759 / NIDDK NIH HHS
Language: English
Date published: 03/01/2004
Academic Unit: Anatomy and Cell Biology; Craniofacial Anomalies Research Center; Health, Sport, and Human Physiology ; Ophthalmology and Visual Sciences
Record Identifier: 9984025337502771

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