Journal article
Epidermal growth factor controls smooth muscle alpha-isoactin expression in BC3H1 cells
The Journal of cell biology, Vol.106(3), pp.797-803
03/1988
DOI: 10.1083/jcb.106.3.797
PMCID: PMC2115111
PMID: 3279054
Abstract
We have examined the effects of epidermal growth factor (EGF), platelet-derived growth factor, and insulin on the differentiation of a mouse vascular smooth muscle-like cell line, the BC3H1 cells. On the basis of cell morphology and smooth muscle alpha-isoactin synthesis, we demonstrate that EGF at physiological concentrations prevents the differentiation of these cells, whereas platelet-derived growth factor has no apparent effect. The induction of alpha-isoactin synthesis by serum deprivation is inhibited by EGF in a dose-dependent manner with a half-maximal effect at 3-5 ng/ml and a maximal inhibition at approximately 30 ng/ml. Northern analysis also shows that EGF blocks the accumulation of alpha-isoactin mRNA normally observed during cell differentiation. Addition of EGF to differentiated cells results in a repression of alpha-isoactin synthesis, a stimulation of beta- and gamma-isoactin synthesis, and the stabilization of the nonmuscle isoactins. The synthesis of creatine phosphokinase, a muscle-specific noncontractile protein, is also regulated by EGF in a similar fashion. Modulation by EGF of alpha-isoactin expression is not affected by aphidicolin and is therefore independent of its mitogenic effect on these cells. Insulin is not required for observation of the EGF-dependent effects but instead seems to promote differentiation. Our results show that EGF can replace serum in controlling the differentiation of BC3H1 cells.
Details
- Title: Subtitle
- Epidermal growth factor controls smooth muscle alpha-isoactin expression in BC3H1 cells
- Creators
- Y C Wang - Department of Biochemistry, University of Iowa College of Medicine, Iowa City 55242P A Rubenstein
- Resource Type
- Journal article
- Publication Details
- The Journal of cell biology, Vol.106(3), pp.797-803
- Publisher
- United States
- DOI
- 10.1083/jcb.106.3.797
- PMID
- 3279054
- PMCID
- PMC2115111
- ISSN
- 0021-9525
- eISSN
- 1540-8140
- Grant note
- HL-14388 / NHLBI NIH HHS
- Language
- English
- Date published
- 03/1988
- Academic Unit
- Stead Family Department of Pediatrics; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984024418902771
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