Epigenetic Modifications of Histones in Periodontal Disease

M D Martins; Yizu Jiao; Lennart Larsson; L O Almeida; Carlos Garaicoa Pazmino; J M Le; C H Squarize; Naohiro Inohara; William V Giannobile; R M Castilho

doi:10.1177/0022034515611876

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Epigenetic Modifications of Histones in Periodontal Disease

Journal article

Peer reviewed

Epigenetic Modifications of Histones in Periodontal Disease

M D Martins, Yizu Jiao, Lennart Larsson, L O Almeida, Carlos Garaicoa Pazmino, J M Le, C H Squarize, Naohiro Inohara, William V Giannobile and R M Castilho

Journal of dental research, Vol.95(2), pp.215-222

02/01/2016

DOI: 10.1177/0022034515611876

PMID: 26496800

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Abstract

Periodontitis is a chronic infectious disease driven by dysbiosis, an imbalance between commensal bacteria and the host organism. Periodontitis is a leading cause of tooth loss in adults and occurs in about 50% of the US population. In addition to the clinical challenges associated with treating periodontitis, the progression and chronic nature of this disease seriously affect human health. Emerging evidence suggests that periodontitis is associated with mechanisms beyond bacteria-induced protein and tissue degradation. Here, we hypothesize that bacteria are able to induce epigenetic modifications in oral epithelial cells mediated by histone modifications. In this study, we found that dysbiosis in vivo led to epigenetic modifications, including acetylation of histones and downregulation of DNA methyltransferase 1. In addition, in vitro exposure of oral epithelial cells to lipopolysaccharides resulted in histone modifications, activation of transcriptional coactivators, such as p300/CBP, and accumulation of nuclear factor-κB (NF-κB). Given that oral epithelial cells are the first line of defense for the periodontium against bacteria, we also evaluated whether activation of pathogen recognition receptors induced histone modifications. We found that activation of the Toll-like receptors 1, 2, and 4 and the nucleotide-binding oligomerization domain protein 1 induced histone acetylation in oral epithelial cells. Our findings corroborate the emerging concept that epigenetic modifications play a role in the development of periodontitis.

Acetylation

Alveolar Bone Loss - microbiology

Animals

Cell Line

Disease Models, Animal

DNA (Cytosine-5-)-Methyltransferase 1

DNA (Cytosine-5-)-Methyltransferases - analysis

Dysbiosis - genetics

Epigenesis, Genetic - genetics

Epithelial Cells - metabolism

Epithelial Cells - microbiology

Fusobacterium nucleatum - genetics

Fusobacterium nucleatum - physiology

Gingival Recession - microbiology

Histones - genetics

Host-Pathogen Interactions - genetics

Humans

Keratinocytes - metabolism

Keratinocytes - microbiology

Lipopolysaccharides - pharmacology

Mice

Mouth Mucosa - cytology

Mouth Mucosa - microbiology

NF-kappa B - analysis

Nod1 Signaling Adaptor Protein - analysis

p300-CBP Transcription Factors - analysis

Periodontal Attachment Loss - microbiology

Periodontitis - genetics

Periodontitis - microbiology

Protein Modification, Translational - genetics

Toll-Like Receptor 1 - analysis

Toll-Like Receptor 2 - analysis

Toll-Like Receptor 4 - analysis

Details

Title: Subtitle: Epigenetic Modifications of Histones in Periodontal Disease
Creators: M D Martins
Yizu Jiao
Lennart Larsson
L O Almeida
Carlos Garaicoa Pazmino
J M Le
C H Squarize
Naohiro Inohara
William V Giannobile
R M Castilho
Resource Type: Journal article
Publication Details: Journal of dental research, Vol.95(2), pp.215-222
DOI: 10.1177/0022034515611876
PMID: 26496800
ISSN: 0022-0345
eISSN: 1544-0591
Language: English
Date published: 02/01/2016
Academic Unit: Periodontics
Record Identifier: 9984130701702771

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