Epithelial Proinflammatory Response and Curcumin-Mediated Protection from Staphylococcal Toxic Shock Syndrome Toxin-1

Matthew M Schaefers; Laura M Breshears; Michele J Anderson; Ying-Chi Lin; Alex E Grill; Jayanth Panyam; Peter J Southern; Patrick M Schlievert; Marnie L Peterson

doi:10.1371/journal.pone.0032813

Back

Epithelial Proinflammatory Response and Curcumin-Mediated Protection from Staphylococcal Toxic Shock Syndrome Toxin-1

Journal article

Open access

Peer reviewed

Epithelial Proinflammatory Response and Curcumin-Mediated Protection from Staphylococcal Toxic Shock Syndrome Toxin-1

Matthew M Schaefers, Laura M Breshears, Michele J Anderson, Ying-Chi Lin, Alex E Grill, Jayanth Panyam, Peter J Southern, Patrick M Schlievert and Marnie L Peterson

PloS one, Vol.7(3), pp.e32813-e32813

03/14/2012

DOI: 10.1371/journal.pone.0032813

PMCID: PMC3303796

PMID: 22431984

Files and links (1)

url

https://doi.org/10.1371/journal.pone.0032813View

Published (Version of record) Open Access

Abstract

Staphylococcus aureus initiates infections and produces virulence factors, including superantigens (SAgs), at mucosal surfaces. The SAg, Toxic Shock Syndrome Toxin-1 (TSST-1) induces cytokine secretion from epithelial cells, antigen presenting cells (APCs) and T lymphocytes, and causes toxic shock syndrome (TSS). This study investigated the mechanism of TSST-1-induced secretion of proinflammatory cytokines from human vaginal epithelial cells (HVECs) and determined if curcumin, an anti-inflammatory agent, could reduce TSST-1-mediated pathology in a rabbit vaginal model of TSS. TSST-1 caused a significant increase in NF-κB-dependent transcription in HVECs that was associated with increased expression of TNF- α, MIP-3α, IL-6 and IL-8. Curcumin, an antagonist of NF-κB-dependent transcription, inhibited IL-8 production from ex vivo porcine vaginal explants at nontoxic doses. In a rabbit model of TSS, co-administration of curcumin with TSST-1 intravaginally reduced lethality by 60% relative to 100% lethality in rabbits receiving TSST-1 alone. In addition, TNF-α was undetectable from serum or vaginal tissue of curcumin treated rabbits that survived. These data suggest that the inflammatory response induced at the mucosal surface by TSST-1 is NF-κB dependent. In addition, the ability of curcumin to prevent TSS in vivo by co-administration with TSST-1 intravaginally suggests that the vaginal mucosal proinflammatory response to TSST-1 is important in the progression of mTSS.

Medicine

Details

Title: Subtitle: Epithelial Proinflammatory Response and Curcumin-Mediated Protection from Staphylococcal Toxic Shock Syndrome Toxin-1
Creators: Matthew M Schaefers - The University of Hong Kong, Hong Kong
Laura M Breshears - The University of Hong Kong, Hong Kong
Michele J Anderson - The University of Hong Kong, Hong Kong
Ying-Chi Lin - The University of Hong Kong, Hong Kong
Alex E Grill - The University of Hong Kong, Hong Kong
Jayanth Panyam - The University of Hong Kong, Hong Kong
Peter J Southern - The University of Hong Kong, Hong Kong
Patrick M Schlievert - The University of Hong Kong, Hong Kong
Marnie L Peterson - The University of Hong Kong, Hong Kong
Resource Type: Journal article
Publication Details: PloS one, Vol.7(3), pp.e32813-e32813
DOI: 10.1371/journal.pone.0032813
PMID: 22431984
PMCID: PMC3303796
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science; San Francisco, USA
Alternative title: Curcumin Stops Staphylococcal Toxic Shock Syndrome
Language: English
Date published: 03/14/2012
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984001216402771

Metrics

29 Record Views

16 Times Cited - Web of Science