Journal article
Epitopes that span the tau molecule are shared with paired helical filaments
Neuron (Cambridge, Mass.), Vol.1(9), pp.817-825
11/1988
DOI: 10.1016/0896-6273(88)90129-8
PMID: 2483104
Abstract
Tau protein has been shown to be an integral component of Alzheimer paired helical filaments (PHF). However, the extent to which tau is incorporated into PHF has not been clear because the antibodies used to label PHF generally do not have precisely defined epitopes. Here we define the antigenic sites for five monoclonal antibodies that react with tau and cross-react with SDS-extracted neurofibrillary tangles. The reactive sites were determined by screening a λgt11 sublibrary expressing small fragments of the tau sequence. The mapped epitopes were found to span almost the entire length of tau, suggesting that PHF contains tau in its entirety or nearly in its entirety. One antibody was found to cross-react with microtubule-associated protein 2, implying some degree of homology between the two proteins.
Details
- Title: Subtitle
- Epitopes that span the tau molecule are shared with paired helical filaments
- Creators
- K.S Kosik - Center for Neurologic Diseases Department of Medicine (Division of Neurology) Brigham and Women's Hospital and Department of Neurology (Neuroscience) Harvard Medical School Boston, Massachusetts 02115, USAL.D Orecchio - Center for Neurologic Diseases Department of Medicine (Division of Neurology) Brigham and Women's Hospital and Department of Neurology (Neuroscience) Harvard Medical School Boston, Massachusetts 02115, USAL Binder - Department of Cell Biology and Anatomy School of Medicine and Dentistry University of Alabama at Birmingham Birmingham, Alabama 35294, USAJ.Q Trojanowski - Department of Pathology and Laboratory Medicine and the University of Pennsylvania School of Medicine Philadelphia, Pennsylvania 19104, uSAV.M.-Y Lee - Department of Pathology and Laboratory Medicine and the University of Pennsylvania School of Medicine Philadelphia, Pennsylvania 19104, uSAG Lee - Center for Neurologic Diseases Department of Medicine (Division of Neurology) Brigham and Women's Hospital and Department of Neurology (Neuroscience) Harvard Medical School Boston, Massachusetts 02115, USA
- Resource Type
- Journal article
- Publication Details
- Neuron (Cambridge, Mass.), Vol.1(9), pp.817-825
- DOI
- 10.1016/0896-6273(88)90129-8
- PMID
- 2483104
- NLM abbreviation
- Neuron
- ISSN
- 0896-6273
- eISSN
- 1097-4199
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 11/1988
- Academic Unit
- Iowa Neuroscience Institute; Immunology; Internal Medicine
- Record Identifier
- 9984065379502771
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