Journal article
Equine Immunoglobulin and Equine Neutralizing F(ab')₂ Protect Mice from West Nile Virus Infection
Viruses, Vol.8(12), pp.332-332
12/18/2016
DOI: 10.3390/v8120332
PMCID: PMC5192393
PMID: 27999340
Abstract
West Nile virus (WNV) is prevalent in Africa, Europe, the Middle East, West Asia, and North America, and causes epidemic encephalitis. To date, no effective therapy for WNV infection has been developed; therefore, there is urgent need to find an efficient method to prevent WNV disease. In this study, we prepared and evaluated the protective efficacy of immune serum IgG and pepsin-digested F(ab')₂ fragments from horses immunized with the WNV virus-like particles (VLP) expressing the WNV M and E proteins. Immune equine F(ab')₂ fragments and immune horse sera efficiently neutralized WNV infection in tissue culture. The passive transfer of equine immune antibodies significantly accelerated the virus clearance in the spleens and brains of WNV infected mice, and reduced mortality. Thus, equine immunoglobulin or equine neutralizing F(ab')₂ passive immunotherapy is a potential strategy for the prophylactic or therapeutic treatment of patients infected with WNV.
Details
- Title: Subtitle
- Equine Immunoglobulin and Equine Neutralizing F(ab')₂ Protect Mice from West Nile Virus Infection
- Creators
- Jiannan Cui - Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China. cjn052015@163.comYongkun Zhao - Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, China. zhaoyongkun1976@126.comHualei Wang - Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, China. wangh25@hotmail.comBoning Qiu - College of Veterinary Medicine, Jilin University, Changchun 130062, China. zerg1230@163.comZengguo Cao - Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China. CC_xiaocao9ye@163.comQian Li - Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China. linglong662222@163.comYanbo Zhang - Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China. zhaoyongkun1976@126.comFeihu Yan - Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China. yanfeihu62041@126.comHongli Jin - College of Veterinary Medicine, Jilin University, Changchun 130062, China. jin8616771@163.comTiecheng Wang - Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, China. wgcha@163.comWeiyang Sun - Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, China. sunweiyang1987@163.comNa Feng - Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, China. fengna0308@126.comYuwei Gao - Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, China. gaoyuwei@gmail.comJing Sun - First Affiliated Hospital of Guangzhou Medical UniversityYanqun Wang - First Affiliated Hospital of Guangzhou Medical UniversityStanley Perlman - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA. stanley-perlman@uiowa.eduJincun Zhao - First Affiliated Hospital of Guangzhou Medical UniversitySongtao Yang - Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, China. yst62041@163.comXianzhu Xia - Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, China. xiaxzh@cae.cn
- Resource Type
- Journal article
- Publication Details
- Viruses, Vol.8(12), pp.332-332
- DOI
- 10.3390/v8120332
- PMID
- 27999340
- PMCID
- PMC5192393
- NLM abbreviation
- Viruses
- ISSN
- 1999-4915
- eISSN
- 1999-4915
- Publisher
- Switzerland
- Language
- English
- Date published
- 12/18/2016
- Academic Unit
- Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
- Record Identifier
- 9983777470702771
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