ErbB-2 via PYK2 upregulates the adhesive ability of androgen receptor-positive human prostate cancer cells

T. C Yuan; F. F Lin; S Veeramani; S. J Chen; H. S Earp; M. F Lin

doi:10.1038/sj.onc.1210570

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ErbB-2 via PYK2 upregulates the adhesive ability of androgen receptor-positive human prostate cancer cells

Journal article

Peer reviewed

ErbB-2 via PYK2 upregulates the adhesive ability of androgen receptor-positive human prostate cancer cells

T. C Yuan, F. F Lin, S Veeramani, S. J Chen, H. S Earp and M. F Lin

Oncogene, Vol.26(54), pp.7552-7559

2007

DOI: 10.1038/sj.onc.1210570

PMID: 17563746

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Abstract

Aberrant regulation in the adhesive ability of cancer cells is closely associated with their metastatic activity. In this study, we examine the role of ErbB-2 in regulating the adhesive ability of androgen receptor (AR)-positive human prostate cancer (PCa) cells, the major cell population of PCa. Utilizing different LNCaP and MDA PCa2b cells as model systems, we found that ErbB-2 activity was correlated with PYK2 activity and adhesive ability in those cells. Increased ErbB-2 expression or activity in LNCaP C-33 cells enhanced PYK2 activation and cell adhesion, while the high PYK2 activity and the rapid adhesion of LNCaP C-81 cells were decreased by diminishing ErbB-2 expression or activity. Knockdown studies revealed the predominant role of ErbB-2 in regulating LNCaP C-81 cell adhesion. Coimmunoprecipitation showed that C-81 cells had increased interaction between ErbB-2 and PYK2. Elevated ErbB-2 activity in LNCaP cells correlated with increased ERK/MAPK activity and enhanced adhesive ability, which were abolished by the expression of K457A-PYK2 mutant or the treatment of PD98059, a MEK inhibitor. In summary, our data suggested that ErbB-2, via PYK2-ERK/MAPK, upregulates the adhesive ability of AR-positive human PCa cells.

Cell Physiology

Tumors

Biological and medical sciences

Cell receptors

Cell structures and functions

Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes

Fundamental and applied biological sciences. Psychology

Gynecology. Andrology. Obstetrics

Male genital diseases

Medical sciences

Miscellaneous

Molecular and cellular biology

Details

Title: Subtitle: ErbB-2 via PYK2 upregulates the adhesive ability of androgen receptor-positive human prostate cancer cells
Creators: T. C Yuan - University of Nebraska Medical Center
F. F Lin - University of Nebraska Medical Center
S Veeramani - University of Nebraska Medical Center
S. J Chen - University of Nebraska Medical Center
H. S Earp - Eppley Institute for Research in Cancer and Allied Diseases
M. F Lin - University of Nebraska Medical Center
Resource Type: Journal article
Publication Details: Oncogene, Vol.26(54), pp.7552-7559
Publisher: Nature Publishing
DOI: 10.1038/sj.onc.1210570
PMID: 17563746
ISSN: 0950-9232
eISSN: 1476-5594
Language: English
Date published: 2007
Academic Unit: Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
Record Identifier: 9984359702802771

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