Journal article
ErbB-4 and TNF-alpha converting enzyme localization to membrane microdomains
Biochemical and biophysical research communications, Vol.350(3), pp.629-633
11/24/2006
DOI: 10.1016/j.bbrc.2006.09.095
PMCID: PMC1637093
PMID: 17027649
Abstract
Sequential proteolytic processing of ErbB-4 occurs in response to ligand addition. Here, we assess the localization of cleavable and non-cleavable ErbB-4 isoforms to membrane microdomains using three methodologies: (1) Triton X-100-insolubility, (2) Brij98-insolubility, and (3) detergent-free density gradient centrifugation. Whereas ErbB-4 translocated to a Triton X-100-insoluble fraction upon treatment of T47D cells with heregulin, it constitutively associated with a Brij98-insoluble fraction and a lipid raft fraction isolated using detergent-free methodology. Comparison of cleavable and non-cleavable isoforms of ErbB-4 revealed that both ErbB-4 isoforms are constitutively localized to either a Triton X-100-soluble or Brij98-insoluble fraction. In contrast, addition of heregulin resulted in translocation of the cleavable isoform to a detergent-free lipid raft. Tumor necrosis factor-a converting enzyme (TACE), the ectodomain secretase for ErbB-4. was present predominantly in its mature active form in most microdomains analyzed. These data suggest the assembly of ErbB-4 ectodomain cleavage apparatus in a membrane microdomain. (c) 2006 Elsevier Inc. All rights reserved.
Details
- Title: Subtitle
- ErbB-4 and TNF-alpha converting enzyme localization to membrane microdomains
- Creators
- Kristina W. Thiel - Vanderbilt UniversityGraham Carpenter - Vanderbilt University
- Resource Type
- Journal article
- Publication Details
- Biochemical and biophysical research communications, Vol.350(3), pp.629-633
- DOI
- 10.1016/j.bbrc.2006.09.095
- PMID
- 17027649
- PMCID
- PMC1637093
- NLM abbreviation
- Biochem Biophys Res Commun
- ISSN
- 0006-291X
- eISSN
- 1090-2104
- Publisher
- Elsevier
- Number of pages
- 5
- Grant note
- T32 CA009582; R01 CA97456; R01 CA097456 / NCI NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) T32CA009582 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
- Language
- English
- Date published
- 11/24/2006
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9984383282602771
Metrics
6 Record Views