Journal article
EspF(U), a type III-translocated effector of actin assembly, fosters epithelial association and late-stage intestinal colonization by E-coli O157 : H7
Cellular microbiology, Vol.10(4), pp.836-847
04/01/2008
DOI: 10.1111/j.1462-5822.2007.01087.x
PMCID: PMC2504705
PMID: 18067584
Abstract
Enterohaemorrhagic Escherichia coli ( EHEC) O157: H7 induces filamentous actin-rich ` pedestals' on intestinal epithelial cells. Pedestal formation in vitro requires translocation of bacterial effectors into the host cell, including Tir, an EHEC receptor, and EspF(U), which increases the efficiency of actin assembly initiated by Tir. While inactivation of espF(U) does not alter colonization in two reservoir hosts, we utilized two disease models to explore the significance of EspF(U)-promoted actin pedestal formation. EHEC Delta espF(U) efficiently colonized the rabbit intestine during co-infection with wild-type EHEC, but co-infection studies on cultured cells suggested that EspFU produced by wild-type bacteria might have rescued the mutant. Significantly, EHEC Delta espF(U) by itself was fully capable of establishing colonization at 2 days post inoculation but unlike wild type, failed to expand in numbers in the caecum and colon by 7 days. In the gnotobiotic piglet model, an espF(U) deletion mutant appeared to generate actin pedestals with lower efficiency than wild type. Furthermore, aggregates of the mutant occupied a significantly smaller area of the intestinal epithelial surface than those of the wild type. Together, these findings suggest that, after initial EHEC colonization of the intestinal surface, EspF(U) may stabilize bacterial association with the epithelial cytoskeleton and promote expansion beyond initial sites of infection.
Details
- Title: Subtitle
- EspF(U), a type III-translocated effector of actin assembly, fosters epithelial association and late-stage intestinal colonization by E-coli O157 : H7
- Creators
- Jennifer M. Ritchie - Brigham and Women's HospitalMichael J. Brady - University of Massachusetts Chan Medical SchoolKathleen N. Riley - Tufts UniversityTheresa Deland Ho - Brigham and Women's HospitalKenneth G. Campellone - University of Massachusetts Chan Medical SchoolIra M. Herman - Tufts UniversityArthur Donohue-Rolfe - Tufts UniversitySaul Tzipori - Tufts UniversityMatthew K. Waldor - Brigham and Women's HospitalJohn M. Leong - University of Massachusetts Chan Medical School
- Resource Type
- Journal article
- Publication Details
- Cellular microbiology, Vol.10(4), pp.836-847
- DOI
- 10.1111/j.1462-5822.2007.01087.x
- PMID
- 18067584
- PMCID
- PMC2504705
- NLM abbreviation
- Cell Microbiol
- ISSN
- 1462-5814
- eISSN
- 1462-5822
- Publisher
- Wiley
- Number of pages
- 12
- Grant note
- R01AI049740 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) P30 DK034928; P30DK-34928 / NIDDK NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) R01 AI049740-04; R21-AI67827; R01 AI046454; R21 AI067827; R01 AI046454-08; R01-AI49470; R01 AI049470 / NIAID NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) R01-A146454 / PHS HHS; United States Department of Health & Human Services; United States Public Health Service P30DK034928 / NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
- Language
- English
- Date published
- 04/01/2008
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984297436002771
Metrics
6 Record Views