Journal article
Essential roles of Gα12/13 signaling in distinct cell behaviors driving zebrafish convergence and extension gastrulation movements
The Journal of cell biology, Vol.169(5), pp.777-787
06/06/2005
DOI: 10.1083/jcb.200501104
PMCID: PMC2171618
PMID: 15928205
Abstract
Gα12/13 have been implicated in numerous cellular processes, however, their roles in vertebrate gastrulation are largely unknown. Here, we show that during zebrafish gastrulation, suppression of both Gα12 and Gα13 signaling by overexpressing dominant negative proteins and application of antisense morpholino-modified oligonucleotide translation interference disrupted convergence and extension without changing embryonic patterning. Analyses of mesodermal cell behaviors revealed that Gα12/13 are required for cell elongation and efficient dorsalward migration during convergence independent of noncanonical Wnt signaling. Furthermore, Gα12/13 function cell-autonomously to mediate mediolateral cell elongation underlying intercalation during notochord extension, likely acting in parallel to noncanonical Wnt signaling. These findings provide the first evidence that Gα12 and Gα13 have overlapping and essential roles in distinct cell behaviors that drive vertebrate gastrulation.
Details
- Title: Subtitle
- Essential roles of Gα12/13 signaling in distinct cell behaviors driving zebrafish convergence and extension gastrulation movements
- Creators
- Fang Lin - Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232Diane S Sepich - Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235Songhai Chen - Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232Jacek Topczewski - Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235, Department of Pediatrics, Northwestern University, Feinberg School of Medicine, Children's Memorial Institute for Education and Research, Chicago, IL 60614Chunyue Yin - Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235Lilianna Solnica-Krezel - Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235Heidi Hamm - Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232
- Resource Type
- Journal article
- Publication Details
- The Journal of cell biology, Vol.169(5), pp.777-787
- DOI
- 10.1083/jcb.200501104
- PMID
- 15928205
- PMCID
- PMC2171618
- NLM abbreviation
- J Cell Biol
- ISSN
- 0021-9525
- eISSN
- 1540-8140
- Language
- English
- Date published
- 06/06/2005
- Academic Unit
- Anatomy and Cell Biology; Iowa Neuroscience Institute; Craniofacial Anomalies Research Center; Fraternal Order of Eagles Diabetes Research Center; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984040536302771
Metrics
14 Record Views