Journal article
Establishment of a Hydrogen Peroxide Resistant Variant of Renal Tubular Epithelial Cells: Role of Calcium-Independent Phospholipase A 2 in Cell Damage
Archives of biochemistry and biophysics, Vol.301(1), pp.119-128
1993
DOI: 10.1006/abbi.1993.1123
Abstract
Renal epithelial cells resistant to oxidant stress mediated by hydrogen peroxide have been isolated and characterized. African green monkey kidney epithelial cell line, BSC-1 cells, chronically exposed to 50 μM hydrogen peroxide for 15 passages exhibited increased catalase (1.5-fold) and glutathione peroxidase (2.4-fold) activity, as well as increased total cellular glutathione (1.6-fold). This was associated with the acquisition of resistance to hydrogen peroxide cytotoxicity, as judged by nuclear staining with ethidium homodimer and clonogenic survival assay. H
2O
2-adapted and wild-type BSC1 cells were used to examine the role of elevated cytosolic calcium concentration and the activation of phospholipase A
2 in the development of lethal cell injury. Despite dramatic differences in resistance to oxidative stress, both cell types showed similar kinetics of cytosolic calcium increase in response to challenge with hydrogen peroxide. In contrast to this, oxidant-induced release of arachidonic acid correlated with the resistance of both types of BSC- 1 cells to oxidative stress. A mechanism-based inhibitor of calcium-independent phospholipase A
2 (
Hazen et al., J. Biol. Chem. 266, 7227, 1991) reduced oxidant-induced lethal cell injury, suggesting that this class of phospholipases contributes to damage of BSC-1 cells exposed to hydrogen peroxide. H
2O
2-adapted BSC1 cells may represent a valuable tool to study adaptation to oxidative stress and various mechanisms of cell injury.
Details
- Title: Subtitle
- Establishment of a Hydrogen Peroxide Resistant Variant of Renal Tubular Epithelial Cells: Role of Calcium-Independent Phospholipase A 2 in Cell Damage
- Creators
- M.S. Goligorsky - State University of New YorkM.A. MorganS. LyubskyR.W. GrossD.T. AdamsD.R. Spitz
- Resource Type
- Journal article
- Publication Details
- Archives of biochemistry and biophysics, Vol.301(1), pp.119-128
- Publisher
- Elsevier Inc
- DOI
- 10.1006/abbi.1993.1123
- ISSN
- 0003-9861
- eISSN
- 1096-0384
- Language
- English
- Date published
- 1993
- Academic Unit
- Pathology; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984312986302771
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