Estimation of adult and neonatal RBC lifespans in anemic neonates using RBCs labeled at several discrete biotin densities

Denison J Kuruvilla; John A Widness; Demet Nalbant; Robert L Schmidt; Donald M Mock; Guohua An; Peter Veng-Pedersen

doi:10.1038/pr.2017.14

Back

Estimation of adult and neonatal RBC lifespans in anemic neonates using RBCs labeled at several discrete biotin densities

Journal article

Peer reviewed

Estimation of adult and neonatal RBC lifespans in anemic neonates using RBCs labeled at several discrete biotin densities

Denison J Kuruvilla, John A Widness, Demet Nalbant, Robert L Schmidt, Donald M Mock, Guohua An and Peter Veng-Pedersen

Pediatric research, Vol.81(6), pp.905-910

06/2017

DOI: 10.1038/pr.2017.14

PMCID: PMC5470643

PMID: 28099421

Files and links (1)

url

http://doi.org/10.1038/pr.2017.14View

Open Access

Abstract

Prior conclusions that autologous neonatal red blood cells (RBC) have substantially shorter lifespans than allogeneic adult RBCs were not based on direct comparison of autologous neonatal vs. allogeneic adult RBCs performed concurrently in the same infant. Biotin labeling of autologous neonatal RBCs and allogeneic adult donor RBCs permits concurrent direct comparison of autologous vs. allogeneic RBC lifespan. RBCs from 15 allogeneic adult donors and from 15 very-low-birth-weight (VLBW) neonates were labeled at separate biotin densities and transfused simultaneously into the 15 neonates. Two mathematical models that account for the RBC differences were employed to estimate lifespans for the two RBC populations. Mean ± SD lifespan for adult allogeneic RBC was 70.1 ± 19.1 d, which is substantially shorter than the 120 d lifespan of both autologous and adult allogeneic RBC in healthy adults. Mean ± SD lifespan for neonatal RBC was 54.2 ± 11.3 d, which is only about 30% shorter than that of the adult allogeneic RBCs. This study provides evidence that extrinsic environmental factors primarily determine RBC survival (e.g., small bore of the capillaries of neonates, rate of oxygenation/deoxygenation cycles) rather than factors intrinsic to RBC.

Anemia - blood

Humans

Adult

Biotin - metabolism

Female

Male

Erythrocyte Aging

Infant, Newborn

Details

Title: Subtitle: Estimation of adult and neonatal RBC lifespans in anemic neonates using RBCs labeled at several discrete biotin densities
Creators: Denison J Kuruvilla - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, Iowa
John A Widness - Department of Pediatrics, College of Medicine, University of Iowa, Iowa City, Iowa
Demet Nalbant - Department of Pediatrics, College of Medicine, University of Iowa, Iowa City, Iowa
Robert L Schmidt - Department of Pediatrics, College of Medicine, University of Iowa, Iowa City, Iowa
Donald M Mock - Department of Molecular Biology and Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas
Guohua An - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, Iowa
Peter Veng-Pedersen - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, Iowa
Resource Type: Journal article
Publication Details: Pediatric research, Vol.81(6), pp.905-910
DOI: 10.1038/pr.2017.14
PMID: 28099421
PMCID: PMC5470643
NLM abbreviation: Pediatr Res
ISSN: 0031-3998
eISSN: 1530-0447
Publisher: United States
Grant note: UL1 TR000039 / NCATS NIH HHS P01 HL046925 / NHLBI NIH HHS U54 TR001356 / NCATS NIH HHS
Language: English
Date published: 06/2017
Academic Unit: Stead Family Department of Pediatrics; Pharmaceutical Sciences and Experimental Therapeutics
Record Identifier: 9984065311302771

Metrics

25 Record Views

22 Times Cited - Web of Science