Estimation of sensitivity and specificity of several Trypanosoma cruzi antibody assays in blood donors in Argentina

Mirta C Remesar; Cecilia Gamba; Ivana F Colaianni; Mónica Puppo; Paula A Sartor; Edward L Murphy; Torsten B Neilands; María A Ridolfi; M Susana Leguizamón; Silvina Kuperman; Ana E Del Pozo

doi:10.1111/j.1537-2995.2009.02301.x

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Estimation of sensitivity and specificity of several Trypanosoma cruzi antibody assays in blood donors in Argentina

Journal article

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Estimation of sensitivity and specificity of several Trypanosoma cruzi antibody assays in blood donors in Argentina

Mirta C Remesar, Cecilia Gamba, Ivana F Colaianni, Mónica Puppo, Paula A Sartor, Edward L Murphy, Torsten B Neilands, María A Ridolfi, M Susana Leguizamón, Silvina Kuperman, …

Transfusion (Philadelphia, Pa.), Vol.49(11), pp.2352-2358

11/2009

DOI: 10.1111/j.1537-2995.2009.02301.x

PMCID: PMC2841448

PMID: 19903291

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Abstract

The absence of a gold standard test for Trypanosoma cruzi antibodies represents a problem not only for the evaluation of screening tests, but also for appropriate blood donor counseling. The aim of this study was to estimate the sensitivity and specificity of multiple blood donor screening tests for T. cruzi antibodies in Argentina. From June 2006 to March 2007 a sample of 1455 blood donors was recruited from two blood banks in Chaco province, an area of Argentina with highly endemic T. cruzi infection. Samples were tested by three epimastigote lysate enzyme immunoassays (EIAs), one recombinant antigen EIA, two indirect hemagglutination assay (IHA) tests, a particle agglutination assay (PA), and a research trans-sialidase inhibition assay (TIA). Sensitivity and specificity were estimated using latent class analysis (LCA). LCA estimated the consensus prevalence of T. cruzi infection at 24.5%. Interassay correlation was higher among the four EIA tests and TIA compared to IHA tests. Assay sensitivities varied from 96 to 99.7 for different EIAs, 91% for TIA, 84% for PA, and 66 to 74% for IHA tests. Relative to the LCA, assay specificities were from 96% to almost 100%. Based on the comparison of several tests in a large population from an endemic area for T. cruzi infection, our data showed an adequate sensitivity for EIA tests in contrast to PA and IHA assays. The latter tests should no longer be used for blood donor screening.

Argentina

Hemagglutination Tests

Reproducibility of Results

Humans

Glycoproteins - metabolism

Neuraminidase - metabolism

Trypanosoma cruzi - isolation & purification

Antibodies, Protozoan

Biological Assay - methods

Immunoenzyme Techniques

Blood Donors

Chagas Disease - diagnosis

Trypanosoma cruzi - immunology

Details

Title: Subtitle: Estimation of sensitivity and specificity of several Trypanosoma cruzi antibody assays in blood donors in Argentina
Creators: Mirta C Remesar - Hospital de Pediatría Prof. Dr. Juan P. Garrahan, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina. mremesar@garrahan.gov.ar
Cecilia Gamba
Ivana F Colaianni
Mónica Puppo
Paula A Sartor
Edward L Murphy
Torsten B Neilands
María A Ridolfi
M Susana Leguizamón
Silvina Kuperman
Ana E Del Pozo
Resource Type: Journal article
Publication Details: Transfusion (Philadelphia, Pa.), Vol.49(11), pp.2352-2358
DOI: 10.1111/j.1537-2995.2009.02301.x
PMID: 19903291
PMCID: PMC2841448
NLM abbreviation: Transfusion
ISSN: 0041-1132
eISSN: 1537-2995
Publisher: United States
Grant note: P30 MH062246 / NIMH NIH HHS D43 TW000003 / FIC NIH HHS K24 HL075036-05 / NHLBI NIH HHS D43TW00003 / FIC NIH HHS R25 MH064712 / NIMH NIH HHS K24 HL075036 / NHLBI NIH HHS K24-HL-75036 / NHLBI NIH HHS R25MH064712 / NIMH NIH HHS
Language: English
Date published: 11/2009
Academic Unit: Psychiatry; Stead Family Department of Pediatrics
Record Identifier: 9984003437702771

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